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PMID: 3895224 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

On ras gene function in yeast.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 82 ·No. 14 ·1985-07-00 ·Pages 4740-4

Fraenkel DG

Abstract

Saccharomyces cerevisiae contains two RAS genes, RAS1 and RAS2. An insertion mutation in RAS2 (ras2::LEU2) does not affect growth on glucose based media but it does prevent growth on media with pyruvate or other noncarbohydrate carbon sources. This defect is pH sensitive and is most severe at pH 7 and above. The ras2::LEU2 mutation also causes markedly higher levels of glycogen in the derepressed phase of growth after glucose exhaustion. Selection for restoration of growth on pyruvate yields unlinked suppressor mutations. Some of the suppressors also reduce glycogen as well as trehalose (the other reserve carbohydrate in yeast) to levels much lower than those of wild-type strains. These suppressor mutations do not suppress the lethality of ras1 ras2 double mutants. The results indirectly accord with yeast RAS2 governing a G protein activity of adenylate cyclase.

MeSH Terms
Animals Crosses, Genetic Culture Media Diploidy Genes, Fungal Glucose/metabolism Glycogen/analysis Mutation Oncogenes Pyruvates/metabolism Saccharomyces cerevisiae/genetics,growth & development Species Specificity Suppression, Genetic Trehalose/analysis
Chemicals
Culture Media Pyruvates Glycogen Trehalose Glucose
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Fraenkel D G
References (43)
43 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.82.14.4740
Published
1985-07-00
Pages
4740-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC390980
Subset
IM
Grants
NIGMS NIH HHS · 5R01 GM21098 · United States
External Links
PubMed source DOI: 10.1073/pnas.82.14.4740 PMC Full Text View journal details
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