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PMID: 3907625 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Electron transfer to nitrogenase in Klebsiella pneumoniae. nifF gene cloned and the gene product, a flavodoxin, purified.

The Biochemical journal ·Vol. 231 ·No. 3 ·1985-11-01 ·Pages 743-53

Deistung J, Cannon FC, Cannon MC, Hill S, Thorneley RN

Abstract

The nifF gene of Klebsiella pneumoniae was cloned into a multicopy plasmid in order to construct a strain that synthesizes and retains an elevated concentration of the gene product relative to the wild-type strain. Characterization of the isolated flavodoxin, which serves as an electron donor to nitrogenase, shows unambiguously that it is the product of the nifF gene.

MeSH Terms
Amino Acids/analysis Cloning, Molecular Electron Transport Electrophoresis, Polyacrylamide Gel Flavodoxin/genetics,isolation & purification,metabolism Flavoproteins/genetics Klebsiella pneumoniae/enzymology,genetics Nitrogenase/metabolism Plasmids Protein Biosynthesis
Chemicals
Amino Acids Flavodoxin Flavoproteins Nitrogenase
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Deistung J
Cannon F C
Cannon M C
Hill S
Thorneley R N
References (31)
31 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2310743
Published
1985-11-01
Pages
743-53
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152811
Subset
IM
External Links
PubMed source DOI: 10.1042/bj2310743 PMC Full Text View journal details
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