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PMID: 3909104 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

The relationship of regulatory proteins and DNase I hypersensitive sites in the yeast GAL1-10 genes.

Nucleic acids research ·Vol. 13 ·No. 23 ·1985-12-09 ·Pages 8409-23

Lohr D, Hopper JE

Abstract

We have used yeast strains containing a disrupted positive (GAL4) and/or a disrupted negative (GAL80) regulatory gene to investigate the relationship of these regulatory proteins to the hypersensitive sites upstream of their target genes, GAL1-10. We find that neither of these regulatory proteins is required for the formation of the hypersensitive region. There is positive regulatory protein (dependent) binding to a portion of the hypersensitive region when GAL1 and 10 are expressed. However, similar binding can also occur under conditions in which the genes are not expressed. Thus, such binding is necessary but not sufficient for expression of GAL1 and 10 and control of GAL1-10 expression must also include processes which occur subsequent to GAL4/DNA binding. The negative regulatory protein GAL80 plays a significant role in these processes.

MeSH Terms
Binding Sites Chromatin/physiology DNA, Fungal/metabolism DNA-Binding Proteins/metabolism Deoxyribonuclease I/metabolism Fungal Proteins/genetics Galactose/genetics Gene Expression Regulation Glucose/metabolism Repressor Proteins/genetics Saccharomyces cerevisiae/genetics Transcription Factors/genetics
Chemicals
Chromatin DNA, Fungal DNA-Binding Proteins Fungal Proteins Repressor Proteins Transcription Factors Deoxyribonuclease I Glucose Galactose
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Lohr D
Hopper J E
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24 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/13.23.8409
Published
1985-12-09
Pages
8409-23
Language
English
Region
England
NLM ID
0411011
PMCID
PMC322142
Subset
IM
Grants
NCI NIH HHS · CA00911 · United States
NIGMS NIH HHS · GM 27925 · United States
NIGMS NIH HHS · GM27623 · United States
External Links
PubMed source DOI: 10.1093/nar/13.23.8409 PMC Full Text View journal details
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