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PMID: 3910026 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cloning and nucleotide sequence analysis of transfer RNA genes from Mycoplasma mycoides.

The Biochemical journal ·Vol. 232 ·No. 1 ·1985-11-15 ·Pages 223-8

Samuelsson T, Elias P, Lustig F, Guindy YS

Abstract

As part of an investigation of the tRNA genes of Mycoplasma mycoides, two HindIII fragments of mycoplasma DNA comprising 0.4 and 2.5 kilobases (kb), respectively, were cloned in pBR322 and their nucleotide sequences determined. Only one tRNA gene was found in the 0.4 kb fragment, the gene for tRNAArg with the anticodon TCT, while the 2.5 kb fragment contained nine different tRNA genes arranged in a cluster which presumably constitutes a transcriptional unit. The clustered tRNA genes, with their respective anticodons, were as follows: Arg (ACG), Pro (TGG), Ala (TGC), Met (CAT), Ile (CAT), Ser (TGA), fMet (CAT), Asp (GTC), and Phe (GAA).

MeSH Terms
Base Sequence Cloning, Molecular Genes, Bacterial Models, Genetic Mycoplasma mycoides/genetics RNA, Transfer/genetics
Chemicals
RNA, Transfer
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Samuelsson T
Elias P
Lustig F
Guindy Y S
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30 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj2320223
Published
1985-11-15
Pages
223-8
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1152862
Subset
IM
Databases
GENBANK
X03153, X03154
External Links
PubMed source DOI: 10.1042/bj2320223 PMC Full Text View journal details
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