Home LiteratureArticle Details
PMID: 6310512 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Sequence analysis of a cluster of twenty-one tRNA genes in Bacillus subtilis.

Nucleic acids research ·Vol. 11 ·No. 16 ·1983-08-25 ·Pages 5763-74

Green CJ, Vold BS

Abstract

The DNA sequence of a cluster of twenty-one tRNA genes distal to a rRNA gene set in B. subtilis was determined. None of the tRNA genes are repeated in the sequence. The only classes of tRNAs that are not represented are those for cysteine, glutamine, tryptophan, and tyrosine. Three of the tRNA genes in this cluster do not have the 3'-CCA sequence encoded in the gene. There is no RNA polymerase terminator sequence in the region between the 5S gene and the first tRNA gene or within the tRNA gene cluster. A terminator sequence was found directly after the last tRNA gene. This rRNA and tRNA gene cluster probably represents one transcriptional unit. However, there may be an RNA polymerase promoter site within this sequence, which raises some interesting questions concerning the regulation of transcription for these tRNA genes.

MeSH Terms
Bacillus subtilis/genetics Base Sequence DNA Restriction Enzymes DNA-Directed RNA Polymerases/metabolism Genes Genes, Bacterial RNA, Ribosomal/genetics RNA, Transfer/genetics Structure-Activity Relationship
Chemicals
RNA, Ribosomal RNA, Transfer DNA-Directed RNA Polymerases DNA Restriction Enzymes
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Green C J
Vold B S
References (29)
29 references, click to expand
  1. Location of genetic loci of soluble RNA on Bacillus subtilis chromosome.
    Proc Natl Acad Sci U S A. 1966 May;55(5):1095-103 PMID: 4959579
  2. Compilation of tRNA sequences.
    Nucleic Acids Res. 1983 Jan 11;11(1):r1-53 PMID: 6866759
  3. The nucleotide sequence of methionine transfer RNA-M.
    Eur J Biochem. 1970 Jan;12(1):177-94 PMID: 4313980
  4. Analysis of isoaccepting transfer ribonucleic acid species of Bacillus subtilis: chromatographic differences between transfer ribonucleic acids from spores and cells in exponential growth.
    J Bacteriol. 1973 Feb;113(2):825-33 PMID: 4632322
  5. New transfer ribonucleic acid species during sporulation of Bacillus subtilis.
    J Bacteriol. 1975 Mar;121(3):950-8 PMID: 803966
  6. Detection of specific sequences among DNA fragments separated by gel electrophoresis.
    J Mol Biol. 1975 Nov 5;98(3):503-17 PMID: 1195397
  7. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  8. Nucleotide sequence of threonine tRNA from Bacillus subtilis.
    Nucleic Acids Res. 1978 Feb;5(2):537-48 PMID: 416425
  9. Complementary sequences 1700 nucleotides apart form a ribonuclease III cleavage site in Escherichia coli ribosomal precursor RNA.
    Proc Natl Acad Sci U S A. 1978 Aug;75(8):3593-7 PMID: 358189
  10. Structure and organization of the two tRNATyr gene clusters on the E. coli chromosome.
    Cell. 1979 Mar;16(3):523-34 PMID: 378394
  11. Transfer ribonucleic acid synthesis during sporulation and spore outgrowth in Bacillus subtilis studied by two-dimensional polyacrylamide gel electrophoresis.
    J Bacteriol. 1979 Nov;140(2):555-66 PMID: 115846
  12. Regulatory sequences involved in the promotion and termination of RNA transcription.
    Annu Rev Genet. 1979;13:319-53 PMID: 94251
  13. RPC-5 column chromatography for the isolation of DNA fragments.
    Methods Enzymol. 1980;65(1):327-47 PMID: 6246356
  14. Primary structure of AUA-specific isoleucine transfer ribonucleic acid from Escherichia coli.
    Biochemistry. 1980 May 13;19(10):2085-9 PMID: 6990971
  15. Nucleotide sequence of non-initiator methionine tRNA from Bacillus subtilis.
    Nucleic Acids Res. 1980 Oct 10;8(19):4517-20 PMID: 6776489
  16. DNA sequence of a T4 transfer RNA gene cluster.
    J Mol Biol. 1980 May 25;139(3):377-91 PMID: 6255163
  17. A system for shotgun DNA sequencing.
    Nucleic Acids Res. 1981 Jan 24;9(2):309-21 PMID: 6259625
  18. Organization and structure of an E. coli tRNA operon containing seven tRNA genes.
    Cell. 1981 Jan;23(1):239-49 PMID: 6163550
  19. Rapid and simple removal of contaminating RNA from plasmid DNA without the use of RNase.
    Anal Biochem. 1981 May 1;113(1):34-42 PMID: 6168210
  20. Cascades of Sigma factors.
    Cell. 1981 Sep;25(3):582-4 PMID: 6793235
  21. tRNA genes are found between 16S and 23S rRNA genes in Bacillus subtilis.
    Nucleic Acids Res. 1982 Mar 11;10(5):1607-24 PMID: 6280153
  22. Multiple RNA polymerase holoenzymes exert transcriptional specificity in Bacillus subtilis.
    Arch Biochem Biophys. 1982 Apr 1;214(2):772-81 PMID: 6807203
  23. The gene for a spinach chloroplast isoleucine tRNA has a methionine anticodon.
    J Biol Chem. 1982 Oct 10;257(19):11191-4 PMID: 6922132
  24. Nucleotide sequence of a spinach chloroplast isoleucine tRNA.
    J Biol Chem. 1982 Oct 10;257(19):11195-8 PMID: 6985567
  25. Post-transcriptional nucleotide addition is responsible for the formation of the 5' terminus of histidine tRNA.
    Proc Natl Acad Sci U S A. 1982 Nov;79(21):6475-9 PMID: 6292903
  26. Detailed physical mapping of the ribosomal RNA genes of Bacillus subtilis.
    Gene. 1982 Sep;19(2):153-62 PMID: 6293924
  27. Structure and organization of a cluster of sic tRNA genes in the space between tandem ribosomal RNA gene sets in Bacillus subtilis.
    J Biol Chem. 1983 Jan 10;258(1):291-8 PMID: 6294101
  28. Two RNA polymerase sigma factors from Bacillus subtilis discriminate between overlapping promoters for a developmentally regulated gene.
    Nature. 1983 Apr 28;302(5911):800-4 PMID: 6405278
  29. Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis.
    J Mol Biol. 1968 Apr 14;33(1):123-40 PMID: 4967203
Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/11.16.5763
Published
1983-08-25
Pages
5763-74
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326312
Subset
IM
Grants
NIGMS NIH HHS · GM 29231 · United States
Databases
GENBANK
K00637, M10606, X00007
External Links
PubMed source DOI: 10.1093/nar/11.16.5763 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2