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PMID: 6997870 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Codon recognition rules in yeast mitochondria.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 77 ·No. 6 ·1980-06-00 ·Pages 3167-70

Bonitz SG, Berlani R, Coruzzi G, Li M, Macino G, Nobrega FG, Nobrega MP, Thalenfeld BE, Tzagoloff A

Abstract

The mitochondrial genome of Saccharomyces cerevisiae codes for 24 tRNAs. The nucleotide sequences of the tRNA genes suggest a unique set of rules that govern the decoding of the mitochondrial genetic code. The four codons of unmixed fmilies are recognized by single tRNAs that always have a U in the wobble position of the anticodon. The codons of the mixed families are read by two different tRNAs. Codons terminating in a C or U are recognized by tRNAs with a G and codons terminating in a G or A are recognized by tRNAs with a U in the corresponding positions of the anticodons. There are two exceptions to these rules. In the AUN family for isoleucine and methionine, the isoleucine tRNA has a G and the methionine tRNA has a C in the wobble position. The tRNA for the arginine CGN family also has an A in the wobble position of the anticodon. It is of interest that the CGN codons have not been found in the mitochondrial genes sequenced to date. The simplified decoding system of yeast mitochondria allows all the codons to be recognized by only 24 tRNAs.

MeSH Terms
Anticodon/genetics Base Sequence Biological Evolution Chromosome Mapping Codon/genetics DNA, Fungal DNA, Mitochondrial/genetics Genetic Code Mitochondria RNA, Messenger/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/genetics
Chemicals
Anticodon Codon DNA, Fungal DNA, Mitochondrial RNA, Messenger RNA, Transfer
Authors & Affiliations
9 authors, click to expand affiliations / ORCID
Bonitz S G
Berlani R
Coruzzi G
Li M
Macino G
Nobrega F G
Nobrega M P
Thalenfeld B E
Tzagoloff A
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.77.6.3167
Published
1980-06-00
Pages
3167-70
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC349575
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.77.6.3167 PMC Full Text View journal details
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