The base sequence of the nifF gene of Klebsiella pneumoniae and homology of the predicted amino acid sequence of its protein product to other flavodoxins.

The Biochemical journal ·Vol. 232 ·No. 3 ·1985-12-15 ·Pages 891-6

Drummond MH

Abstract

The nucleotide sequence of a 629 base-pair segment of DNA spanning the nifF gene of Klebsiella pneumoniae is presented. The structural gene comprises 531 base-pairs (175 codons, excluding the translational initiator and terminator) encoding an acidic polypeptide of 18950 Da. The nifF product thus belongs to the long-chain class of flavodoxins. It shows some sequence homology to the short-chain flavodoxins from Desulfovibrio vulgaris, Clostridium MP and Megasphaera elsdenii, and much stronger homology to long-chain flavodoxins from Azotobacter vinelandii and Anacystis nidulans. The long chain flavodoxins thus seem to constitute a well-conserved sub-group. The homology with the A. vinelandii flavodoxin is particularly strong, which may reflect their common function in nitrogen fixation.

MeSH Terms

Amino Acid Sequence Base Sequence Flavodoxin/genetics Flavoproteins/genetics Genes, Bacterial Klebsiella pneumoniae/genetics Protein Biosynthesis Species Specificity

Chemicals

Flavodoxin Flavoproteins

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Drummond M H

References (40)

40 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj2320891

Published

1985-12-15

Pages

891-6

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1152966

Subset

Databases

GENBANK

M23868

External Links

PubMed source DOI: 10.1042/bj2320891 PMC Full Text View journal details

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The base sequence of the nifF gene of Klebsiella pneumoniae and homology of the predicted amino acid sequence of its protein product to other flavodoxins.

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