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PMID: 3915532 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Regulation of the transcript for a lysosomal protein: evidence for a gene program modified by platelet-derived growth factor.

Molecular and cellular biology ·Vol. 5 ·No. 10 ·1985-10-00 ·Pages 2582-9

Frick KK, Doherty PJ, Gottesman MM, Scher CD

Abstract

Platelet-derived growth factor (PDGF) stimulates density-arrested BALB/c-3T3 cells to synthesize MEP, a lysosomal protein. This enhanced synthesis appears to be largely regulated by the PDGF-modulated accumulation of MEP mRNA, a 1.8-kilobase species. The increase in the MEP transcript, which is dependent on the PDGF concentration, begins 3 to 4 h after PDGF addition and is maximal at 12 h. The accumulation of the MEP transcript is growth-factor specific: PDGF and the tumor promoter 12-O-tetradecanoylphorbol-13-acetate, an agent which acts like PDGF, induce MEP RNA accumulation, whereas epidermal growth factor, somatomedin C, insulin, and whole plasma do not. A spontaneously transformed BALB/c-3T3 cell line (ST2-3T3), which does not require PDGF for growth, optimally expresses MEP RNA in the absence of PDGF. The PDGF-modulated increase in MEP RNA is unlike PDGF-modulated c-myc and c-fos RNA accumulation because it is blocked by cycloheximide, suggesting a requirement for de novo protein synthesis. It appears that PDGF modulates a program of gene expression with the accumulation of some transcripts, typified by MEP, being dependent upon the translation of others.

MeSH Terms
Animals Cloning, Molecular Cycloheximide/pharmacology DNA/genetics Epidermal Growth Factor/pharmacology Gene Expression Regulation/drug effects Insulin/pharmacology Insulin-Like Growth Factor I/pharmacology Lysosomes/physiology Mice Platelet-Derived Growth Factor/physiology Proteins/genetics RNA, Messenger/genetics
Chemicals
Insulin Platelet-Derived Growth Factor Proteins RNA, Messenger Epidermal Growth Factor Insulin-Like Growth Factor I DNA Cycloheximide
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Frick K K
Doherty P J
Gottesman M M
Scher C D
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.5.10.2582-2589.1985
Published
1985-10-00
Pages
2582-9
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC366993
Subset
IM
Grants
NCI NIH HHS · CA34162 · United States
External Links
PubMed source DOI: 10.1128/mcb.5.10.2582-2589.1985 PMC Full Text View journal details
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