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PMID: 3932821 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Viability of Escherichia coli K-12 DNA adenine methylase (dam) mutants requires increased expression of specific genes in the SOS regulon.

Molecular & general genetics : MGG ·Vol. 201 ·No. 1 ·1985-00-00 ·Pages 14-9

Peterson KR, Wertman KF, Mount DW, Marinus MG

Abstract

We have examined the level of expression of the SOS regulon in cells lacking DNA adenine methylase activity (dam-). Mud (Ap, lac) fusions to several SOS operons (recA, lexA, uvrA, uvrB, uvrD, sulA, dinD and dinF) were found to express higher levels of beta-galactosidase in dam- strains than in isogenic dam+ strains. The attempted construction of dam- strains that were also mutant in one of several SOS genes indicated that the viability of methylase-deficient strains correlates with the inactivation of the SOS repressor (LexA protein). Consistent with this, the wild-type functions of two LexA-repressed genes (recA and ruv) appear to be required for dam- strain viability.

MeSH Terms
DNA Repair DNA, Bacterial/genetics Escherichia coli/enzymology,genetics,growth & development Genes Genes, Bacterial Genotype Methyltransferases/genetics Mutation Rec A Recombinases/genetics Site-Specific DNA-Methyltransferase (Adenine-Specific) Species Specificity Transduction, Genetic beta-Galactosidase/genetics
Chemicals
DNA, Bacterial Methyltransferases Site-Specific DNA-Methyltransferase (Adenine-Specific) Rec A Recombinases beta-Galactosidase
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Peterson K R
Wertman K F
Mount D W
Marinus M G
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66 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00397979
Published
1985-00-00
Pages
14-9
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
Grants
NIGMS NIH HHS · GM24496 · United States
NIGMS NIH HHS · GM30330 · United States
External Links
PubMed source DOI: 10.1007/BF00397979
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