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PMID: 393956 Published · ppublish English Journal Article

DNA repair in Proteus mirabilis. VI. Plasmid (R46-) mediated recovery and UV mutagenesis.

Molecular & general genetics : MGG ·Vol. 176 ·No. 2 ·1979-10-03 ·Pages 265-73

Hofemeister J, Köhler H, Filippov VD

Abstract

The expression of plasmid R46-mediated recovery and mutagenic function (s) was studied in P. mirabilis, which is normally either weakly or non-mutable after UV exposure. The plasmid was found to confer on P. mirabilis enhanced UV resistance as well as UV-induced mutability for various types of forward mutations and reversion of the thr273 mutation. The plasmid enhanced survival of UV-irradiated phages in P. mirabilis both in unirradiated host cells and with increased efficiency after UV-exposure of host cells, as is characteristic of UV-inducible phage reactivation. Spontaneous mutability of P. mirabilis harboring R46 was about 2 to 7 times higher than that of cells without plasmid, depending on the marker, repair type, and plating density of the cells used. All of these R46-mediated rescue and mutagenic functions require the rec672 + gene function. It is assumed that the plasmid R46 adds functions to P. mirabilis comparable to those deficient in umuC and uvm mutants of E. coli (Kato and Shinoura, 1977; Steinborn, 1978) and that P. mirabilis possesses functions homologous to those controlled in E. coli by the recA + and lexA + genes. The significance of plasmid-mediated rescue and mutagenic functions for bacteria which lack the misrepair branch of mutagenesis, is discussed.

MeSH Terms
Animals Bacteriophages/genetics DNA Repair Lysogeny/radiation effects Mutation/radiation effects Plasmids Proteus mirabilis/genetics,radiation effects Recombination, Genetic Ultraviolet Rays
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Hofemeister J
Köhler H
Filippov V D
References (31)
31 references, click to expand
  1. Ultraviolet light protection, enhancement of ultraviolet light mutagenesis, and mutator effect of plasmid R46 in Salmonella typhimurium.
    J Bacteriol. 1976 Oct;128(1):271-82 PMID: 789333
  2. Inducible reactivation and mutagenesis of UV-irradiated bacteriophage P22 in Salmonella typhimurium LT2 containing the plasmid pKM101.
    J Bacteriol. 1978 Aug;135(2):415-21 PMID: 355224
  3. Ultraviolet mutagenesis and inducible DNA repair in Escherichia coli.
    Bacteriol Rev. 1976 Dec;40(4):869-907 PMID: 795416
  4. Uvm mutants of Escherichia coli K12 deficient in UV mutagenesis. II. Further evidence for a novel function in error-prone repair.
    Mol Gen Genet. 1979 Sep;175(2):203-8 PMID: 160001
  5. Increase in frequency of ultraviolet-induced mutation brought about by the colicine factor, col-I in Salmonella typhimurium.
    Mutat Res. 1966 Apr;3(2):129-34 PMID: 5333391
  6. [STUDIES ON UV-INDUCED MUTATION IN SOME STREPTOMYCIN-DEPENDENT STRAINS OF PROTEUS MIRABILIS HAUSER].
    Z Allg Mikrobiol. 1964;4:22-41 PMID: 14298264
  7. The relation of repair phenomena to mutation induction in bacteria.
    Mutat Res. 1978;55(2):85-120 PMID: 372787
  8. DNA repair in Proteus mirabilis. IV. Post-irradiation DNA degradation as influenced by a function inducible in rec+ cells.
    Mol Gen Genet. 1977 Jul 7;154(1):35-41 PMID: 331077
  9. A synthetic medium for the L type colonies of Proteus.
    J Bacteriol. 1954 Nov;68(5):530-3 PMID: 13211552
  10. Plasmid (pKM101)-mediated enhancement of repair and mutagenesis: dependence on chromosomal genes in Escherichia coli K-12.
    Mol Gen Genet. 1977 Mar 28;152(1):93-103 PMID: 325391
  11. Analysis of the role of recombination and repair in mutagenesis of Escherichia coli by UV irradiation.
    Genetics. 1977 Sep;87(1):1-18 PMID: 334628
  12. Expression of the plasmid pKM101--determined DNA repair system in recA- and lex- strains of Escherichia coli.
    Mol Gen Genet. 1976 Jun 15;145(3):303-6 PMID: 781517
  13. Effect of an R factor on resistance of Salmonella typhimurium to radiation and chemical treatment.
    Mutat Res. 1972 Apr;14(4):450-3 PMID: 4552776
  14. The effect of a drug-resistance factor on recombination and repair of DNA in Escherichia coli K12.
    J Gen Microbiol. 1977 Jul;101(1):93-8 PMID: 330823
  15. R (transmissible drug-resistance) factors in Salmonella typhimurium: pattern of transduction by phage P22 and ultraviolet-protection effect.
    J Gen Microbiol. 1968 Aug;53(1):109-23 PMID: 4878051
  16. R factor-mediated resistance to ultraviolet light in strains of Escherichia coli deficient in known repair functions.
    J Gen Microbiol. 1976 Mar;93(1):103-10 PMID: 772160
  17. Induction of protein X in Escherichia coli.
    Mol Gen Genet. 1977 Feb 15;150(3):237-48 PMID: 321932
  18. Restoration of mutability in non-mutable Escherichia coli carrying different plasmids.
    Mol Gen Genet. 1977 Oct 24;155(3):287-90 PMID: 202859
  19. Proteolytic cleavage of bacteriophage lambda repressor in induction.
    Proc Natl Acad Sci U S A. 1975 Jan;72(1):147-51 PMID: 1090931
  20. Detection of carcinogens as mutagens: bacterial tester strains with R factor plasmids.
    Proc Natl Acad Sci U S A. 1975 Mar;72(3):979-83 PMID: 165497
  21. Isolation and characterization of mutants of Escherichia coli deficient in induction of mutations by ultraviolet light.
    Mol Gen Genet. 1977 Nov 14;156(2):121-31 PMID: 340898
  22. Resistance to the bactericidal effect of ultraviolet radiation conferred on Enterobacteria by the colicine factor coli.
    J Gen Microbiol. 1965 Jul;40(1):43-55 PMID: 5323082
  23. Spontaneous, ultraviolet and ionizing radiation mutagenesis in two auxotrophic strains of Salmonella typhimurium carrying an R plasmid.
    Mutat Res. 1977 Oct;45(1):1-6 PMID: 335234
  24. Inducible, error-free DNA Repair in tsl recA mutants of E. coli.
    Mol Gen Genet. 1976 Jul 5;146(1):37-41 PMID: 785206
  25. Effect of R factors and other plasmids on ultraviolet susceptibility and host cell reactivation property of Escherichia coli.
    J Bacteriol. 1969 Oct;100(1):337-46 PMID: 4898998
  26. Effect of rec mutations on the ultraviolet protecting and mutation-enhancing properties of the plasmid R-Utrecht in Salmonella typhimurium.
    Mutat Res. 1973 Sep;19(3):357-9 PMID: 4585477
  27. Uvm mutants of Escherichia coli K12 deficient in UV mutagenesis. I. Isolation of uvm mutants and their phenotypical characterization in DNA repair and mutagenesis.
    Mol Gen Genet. 1978 Sep 20;165(1):87-93 PMID: 362169
  28. R factors improving survival of Escherichia coli K-12 after ultraviolet irradiation.
    J Bacteriol. 1969 Oct;100(1):128-39 PMID: 4898981
  29. DNA repair in Proteus mirabilis. III.Survival, dimer excision, and UV reactivation in comparison with Escherichia coli K12.
    Mol Gen Genet. 1975 Nov 24;141(2):147-61 PMID: 1107808
  30. Spontaneous and ultraviolet-induced mutation in Escherichia coli: interaction between plasmid and tif-i mutator effects.
    J Gen Microbiol. 1977 Jul;101(1):163-6 PMID: 330822
  31. Partial suppression of the LexA phenotype by mutations (rnm) which restore ultraviolet resistance but not ultraviolet mutability to Escherichia coli B/r uvr A lexA.
    Mutat Res. 1976 Jul;36(1):17-28 PMID: 781527
Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
Published
1979-10-03
Pages
265-73
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source
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