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PMID: 3943898 Published · ppublish English Journal Article

Identification and preliminary characterization of a lectinlike protein from Capnocytophaga gingivalis (emended).

Infection and immunity ·Vol. 51 ·No. 2 ·1986-02-00 ·Pages 490-4

Kagermeier A, London J

Abstract

A polypeptide believed to be the monomeric form of the lectin responsible for the coaggregation of Capnocytophaga gingivalis (emended) and Actinomyces israelii has been identified. Denaturing polyacrylamide gel electrophoresis and immunoblot analyses were used to distinguish the protein from other proteins in the outer membrane of C. gingivalis. The subunit of the putative lectin has a pI of 8.6 and a molecular weight of 155,000.

MeSH Terms
Agglutination Animals Bacterial Outer Membrane Proteins/analysis Capnocytophaga/analysis,immunology Cytophagaceae/analysis Electrophoresis, Polyacrylamide Gel Lectins/analysis Molecular Weight Mutation Rabbits
Chemicals
Bacterial Outer Membrane Proteins Lectins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kagermeier A
London J
References (21)
21 references, click to expand
  1. Cell wall-associated protein antigens of Streptococcus salivarius: purification, properties, and function in adherence.
    Infect Immun. 1982 Oct;38(1):233-42 PMID: 7141692
  2. The reliability of molecular weight determinations by dodecyl sulfate-polyacrylamide gel electrophoresis.
    J Biol Chem. 1969 Aug 25;244(16):4406-12 PMID: 5806584
  3. Polypeptides of the tail fibres of bacteriophage T4.
    J Mol Biol. 1971 Dec 28;62(3):465-77 PMID: 5136579
  4. A simplified method for the quantitative assay of small amounts of protein in biologic material.
    Anal Biochem. 1973 Feb;51(2):654-5 PMID: 4735559
  5. High resolution two-dimensional electrophoresis of proteins.
    J Biol Chem. 1975 May 25;250(10):4007-21 PMID: 236308
  6. Two-dimensional gel electrophoresis of membrane proteins.
    Biochemistry. 1976 Feb 10;15(3):616-23 PMID: 766831
  7. Membrane-bound, pyridine nucleotide-independent L-lactate dehydrogenase of Rhodopseudomonas sphaeroides.
    J Bacteriol. 1978 Feb;133(2):593-600 PMID: 304854
  8. Investigations of the pigments from Cytophaga johnsonae Cy jl. New flexirubin-type pigments.
    Arch Microbiol. 1978 Jun 26;117(3):253-7 PMID: 697500
  9. Rapid method for identification and enumeration of oral Actinomyces.
    Infect Immun. 1978 Sep;21(3):786-91 PMID: 711333
  10. Specificity of coaggregation reactions between human oral streptococci and strains of Actinomyces viscosus or Actinomyces naeslundii.
    Infect Immun. 1979 Jun;24(3):742-52 PMID: 468376
  11. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
    Proc Natl Acad Sci U S A. 1979 Sep;76(9):4350-4 PMID: 388439
  12. Ultrasensitive stain for proteins in polyacrylamide gels shows regional variation in cerebrospinal fluid proteins.
    Science. 1981 Mar 27;211(4489):1437-8 PMID: 6162199
  13. Attachment of oral Cytophaga species to hydroxyapatite-containing surfaces.
    Infect Immun. 1980 Aug;29(2):768-77 PMID: 7216436
  14. Identification of a Streptococcus salivarius cell wall component mediating coaggregation with Veillonella alcalescens V1.
    Infect Immun. 1981 May;32(2):723-30 PMID: 7251145
  15. The use of Tween 20 as a blocking agent in the immunological detection of proteins transferred to nitrocellulose membranes.
    J Immunol Methods. 1982 Dec 30;55(3):297-307 PMID: 6820029
  16. Coaggregation of human oral Cytophaga species and Actinomyces israelii.
    Infect Immun. 1983 Jun;40(3):1178-85 PMID: 6133836
  17. Detection of erythrocyte membrane proteins, sialoglycoproteins, and lipids in the same polyacrylamide gel using a double-staining technique.
    Proc Natl Acad Sci U S A. 1984 Mar;81(6):1733-7 PMID: 6200882
  18. Evidence for the participation of N-acetylated amino sugars in the coaggregation between Cytophaga species strain DR2001 and Actinomyces israelii PK16.
    Infect Immun. 1984 May;44(2):299-305 PMID: 6715035
  19. Cell to cell interactions of Capnocytophaga and Bacteroides species with other oral bacteria and their potential role in development of plaque.
    J Periodontal Res. 1984 Nov;19(6):564-9 PMID: 6241228
  20. Coaggregation of oral Bacteroides species with other bacteria: central role in coaggregation bridges and competitions.
    Infect Immun. 1985 Jun;48(3):741-6 PMID: 3888842
  21. Expression of Actinomyces viscosus antigens in Escherichia coli: cloning of a structural gene (fimA) for type 2 fimbriae.
    J Bacteriol. 1985 Jun;162(3):1075-8 PMID: 2860095
Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
Published
1986-02-00
Pages
490-4
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262361
Subset
IM
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