Expression of Actinomyces viscosus antigens in Escherichia coli: cloning of a structural gene (fimA) for type 2 fimbriae.

Journal of bacteriology ·Vol. 162 ·No. 3 ·1985-06-00 ·Pages 1075-8

Donkersloot JA, Cisar JO, Wax ME, Harr RJ, Chassy BM

Abstract

A cosmid gene library of Actinomyces viscosus T14V was prepared in Escherichia coli to examine the expression of A. viscosus antigens and to gain insight into the structure of A. viscosus type 1 and type 2 fimbriae. Out of this library of 550 clones, 28 reacted in a colony immunoassay with antibodies against A. viscosus cells. The proteins responsible for these reactions were identified in three clones. Clones AV1209 and AV2009 displayed nonfimbrial antigens with subunits of 40 and 58 kilodaltons, respectively. Clone AV1402 showed a 59-kilodalton protein that reacted with monospecific antibody against type 2 fimbriae and that comigrated with a subunit of type 2 fimbriae during sodium dodecyl sulfate-polyacrylamide gel electrophoresis. This indicates that AV1402 expresses a gene (fimA) for a subunit of A. viscosus type 2 fimbriae.

MeSH Terms

Actinomyces/genetics,immunology Antigens, Bacterial/analysis Cloning, Molecular Escherichia coli/genetics Fimbriae, Bacterial Genes

Chemicals

Antigens, Bacterial

Authors & Affiliations

5 authors, click to expand affiliations / ORCID

Donkersloot J A

Cisar J O

Wax M E

Harr R J

Chassy B M

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25 references, click to expand

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Article Info

Journal

Journal of bacteriology

Abbr.

J Bacteriol

ISSN

0021-9193

DOI

10.1128/jb.162.3.1075-1078.1985

Published

1985-06-00

Pages

1075-8

Language

English

Region

United States

NLM ID

2985120R

PMCID

PMC215885

Subset

External Links

PubMed source DOI: 10.1128/jb.162.3.1075-1078.1985 PMC Full Text View journal details

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Expression of Actinomyces viscosus antigens in Escherichia coli: cloning of a structural gene (fimA) for type 2 fimbriae.

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