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PMID: 3949385 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Requirement for lipopolysaccharide-responsive macrophages in galactosamine-induced sensitization to endotoxin.

Infection and immunity ·Vol. 51 ·No. 3 ·1986-03-00 ·Pages 891-5

Freudenberg MA, Keppler D, Galanos C

Abstract

Injection of D-galactosamine sensitizes mice many thousand-fold to the lethal action of endotoxin (lipopolysaccharide [LPS]). Comparable sensitization was practically absent in LPS-resistant C3H/HeJ mice, which after D-galactosamine treatment were about 500,000 times less sensitive to LPS lethality than histocompatible LPS-sensitive C3H/HeN mice. D-Galactosamine induces changes in the hepatocytes of treated animals, such as depletion of UTP and alterations in the pattern of UDP sugars. These early biochemical changes, which are necessary for development of sensitization, were similar in both mouse strains which we examined. High sensitivity to the lethal effects of LPS was achieved in C3H/HeJ mice after D-galactosamine treatment by transfer of C3H/HeN macrophages obtained in culture from bone marrow precursor cells.

MeSH Terms
Animals Bacterial Toxins/immunology Endotoxins/immunology,toxicity Female Galactosamine/pharmacology Lipopolysaccharides/immunology Liver/metabolism Macrophage Activation Macrophages/immunology Male Mice Mice, Inbred Strains/immunology Salmonella/immunology Uracil Nucleotides/metabolism
Chemicals
Bacterial Toxins Endotoxins Lipopolysaccharides Uracil Nucleotides Galactosamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Freudenberg M A
Keppler D
Galanos C
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22 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.51.3.891-895.1986
Published
1986-03-00
Pages
891-5
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC260982
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.51.3.891-895.1986 PMC Full Text View journal details
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