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PMID: 4018873 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Marked enhancement in vivo of adjuvant activity of muramyl dipeptide to protein antigens and to synthetic weak immunogens with monoclonal anti-muramyl dipeptide antibodies.

Infection and immunity ·Vol. 49 ·No. 2 ·1985-08-00 ·Pages 312-9

Bahr GM, Tello DS, Chedid LA

Abstract

Priming of mice with complexes of antigen coupled to muramyl dipeptide and monoclonal anti-muramyl dipeptide antibodies enhanced the adjuvant activity of muramyl dipeptide on the humoral response to the antigen. The enhancement did not occur with free (uncoupled) muramyl dipeptide and required the presence of an adjuvant-active hapten within the complex as well as the Fc fragment of the monoclonal antibody. This system proved highly effective in eliciting antibodies to synthetic weak immunogens whereas muramyl dipeptide, on its own, exerts very little or no adjuvant activity. The effect was not due to a general polyclonal activation and was restricted to the antigen coupled to the synthetic adjuvant. Possible pathways involved in this phenomenon are discussed.

MeSH Terms
Acetylmuramyl-Alanyl-Isoglutamine/immunology Adjuvants, Immunologic Animals Antibodies, Monoclonal Antibody Formation Antigen-Antibody Complex Antigens/immunology Enzyme-Linked Immunosorbent Assay Female Haptens Immunoglobulin Fab Fragments Mice Mice, Inbred BALB C
Chemicals
Adjuvants, Immunologic Antibodies, Monoclonal Antigen-Antibody Complex Antigens Haptens Immunoglobulin Fab Fragments Acetylmuramyl-Alanyl-Isoglutamine
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bahr G M
Tello D S
Chedid L A
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35 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.49.2.312-319.1985
Published
1985-08-00
Pages
312-9
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC262016
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.49.2.312-319.1985 PMC Full Text View journal details
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