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PMID: 4068144 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

A new pathway in the generation of defective retrovirus DNA.

Journal of virology ·Vol. 56 ·No. 3 ·1985-12-00 ·Pages 779-89

Olsen JC, Swanstrom R

Abstract

We used a retrovirus shuttle vector to make molecular clones of circular viral DNA from infected cells. One-third of the molecules examined had deletions that started within or near the U5 domain of the long terminal repeat (LTR) region and extended a variable distance toward the gag gene. We present evidence that some of these deletions arose by cleavage of a single LTR unit, in contrast to the cleavage of tandem LTR units associated with the integration reaction. These results suggest that in the formation of defective circular DNA, the U5 domain can be recognized and cleaved in the absence of an adjacent U3 domain. The cleavage of isolated U5 domains may represent an important mechanism responsible for the generation of certain forms of both defective circular DNA and defective integrated DNA.

MeSH Terms
Animals Chick Embryo Chromosome Deletion Chromosome Mapping DNA, Circular/genetics DNA, Viral/genetics Defective Viruses/genetics Genetic Linkage Genetic Vectors Recombination, Genetic Repetitive Sequences, Nucleic Acid Retroviridae/genetics Viral Envelope Proteins/genetics
Chemicals
DNA, Circular DNA, Viral Viral Envelope Proteins
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Olsen J C
Swanstrom R
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.56.3.779-789.1985
Published
1985-12-00
Pages
779-89
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC252648
Subset
IM
Grants
NCI NIH HHS · 5T32 CA09156 · United States
NCI NIH HHS · R01 CA33147 · United States
External Links
PubMed source DOI: 10.1128/JVI.56.3.779-789.1985 PMC Full Text View journal details
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