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PMID: 40882 Published · ppublish English Journal Article Research Support, U.S. Gov't, Non-P.H.S. Research Support, U.S. Gov't, P.H.S.

Attachment pili from enterotoxigenic Escherichia coli pathogenic for humans.

Infection and immunity ·Vol. 26 ·No. 1 ·1979-10-00 ·Pages 362-8

Deneke CF, Thorne GM, Gorbach SL

Abstract

Pili from enterotoxigenic Escherichia coli pathogenic for humans have been isolated by adsorption to the surface of erythrocytes followed by thermal elution. The pili are composed of two protein subunits with molecular weights of 13,100 and 12,500 as determined by sodium dodecyl sulfate-gel electrophoresis. These pili also bind to human buccal cells under temperature conditions (37 degrees C) which prevent the binding of these pili to the erythrocytes. Analogous temperature effects on binding have previously been observed with whole bacterial cells. This binding can be inhibited by antiserum prepared against the isolated pili.

MeSH Terms
Bacterial Proteins/analysis Cheek Enterotoxins/biosynthesis Erythrocytes/metabolism Escherichia coli/ultrastructure Fimbriae, Bacterial/analysis,immunology,metabolism Humans Immunoglobulin G/physiology Mouth Mucosa/metabolism Peptides/analysis
Chemicals
Bacterial Proteins Enterotoxins Immunoglobulin G Peptides
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Deneke C F
Thorne G M
Gorbach S L
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36 references, click to expand
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Article Info
Journal
Infection and immunity
Abbr.
Infect Immun
ISSN
0019-9567
DOI
10.1128/iai.26.1.362-368.1979
Published
1979-10-00
Pages
362-8
Language
English
Region
United States
NLM ID
0246127
PMCID
PMC414620
Subset
IM
External Links
PubMed source DOI: 10.1128/iai.26.1.362-368.1979 PMC Full Text View journal details
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