Home LiteratureArticle Details
PMID: 4099664 Published · ppublish English Journal Article

Histone-acetylating enzyme of brain.

The Biochemical journal ·Vol. 119 ·No. 4 ·1970-10-00 ·Pages 665-72

Bondy SC, Roberts S, Morelos BS

Abstract

1. Acetylation of histones by an enzyme system derived from rat brain and liver (histone acetylase) was studied by using [1-(14)C]acetyl-CoA as the acetyl group donor. 2. The activity of this enzyme was largely confined to the nucleus. 3. Histone-acetylating activity of cerebral nuclei purified by centrifugation through 1.9m-sucrose was not altered by the presence of the cytoplasmic fraction. 4. Cerebral nuclei from adult rats exhibited greater histone-acetylating activity than did the corresponding preparation from newborn animals. 5. Nuclear acetylating activity was higher in brain than in liver of adult rats but not in newborn animals. 6. The partially purified enzyme from cerebral nuclei, prepared by ammonium sulphate fractionation of an acetone-dried powder, specifically catalysed histone acetylation. 7. Polylysine, protamine, serum albumin and gamma-globulin were not enzymically acetylated by this preparation. 8. Soluble acetylating preparations from both brain and liver nuclei were more active towards arginine-rich F3 and slightly lysine-rich F2a and F2b histone fractions than towards the lysine-rich F1 fraction. 9. Enzymic acetylation of chromatin-bound proteins was much less extensive than that of free histones. 10. The high histone acetylase activity in mature brain may reflect the importance of this process in the genetic control of cerebral function.

MeSH Terms
Acetylesterase/analysis Animals Brain/enzymology Carbon Isotopes Cell Nucleus/metabolism Chromosomes Coenzyme A/metabolism Histones/metabolism In Vitro Techniques Liver/enzymology Lysine/metabolism Protamines/metabolism Protein Binding RNA/biosynthesis Rats Serum Albumin/metabolism gamma-Globulins/metabolism
Chemicals
Carbon Isotopes Histones Protamines Serum Albumin gamma-Globulins RNA Acetylesterase Lysine Coenzyme A
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Bondy S C
Roberts S
Morelos B S
References (41)
41 references, click to expand
  1. Turnover of template-bound histone.
    Proc Natl Acad Sci U S A. 1965 Aug;54(2):498-505 PMID: 5217438
  2. RNA synthesis and histone acetylation during the course of gene activation in lymphocytes.
    Proc Natl Acad Sci U S A. 1966 Apr;55(4):805-12 PMID: 5219687
  3. A glial protein specific for the nervous system.
    Proc Natl Acad Sci U S A. 1966 Feb;55(2):354-8 PMID: 4957412
  4. The relationship between cellular nucleic acids in the developing rat cerebral cortex.
    Biochem J. 1966 Feb;98(2):636-40 PMID: 5941354
  5. Metabolism of histones of brain and liver.
    J Biol Chem. 1966 May 25;241(10):2397-404 PMID: 5911618
  6. Rapidly-labelled ribonucleic acids in brain.
    J Neurochem. 1966 Aug;13(8):619-28 PMID: 5911839
  7. The ribonucleic acid metabolism of the brain.
    J Neurochem. 1966 Oct;13(10):955-9 PMID: 5927764
  8. Metabolic integrity of deoxyribonucleohistones.
    J Mol Biol. 1966 Jun;17(2):311-8 PMID: 4289995
  9. Binding of hydrocortisone to rat liver histones.
    J Mol Biol. 1966 Aug;19(2):591-5 PMID: 5969083
  10. The cytoplasmic control of nuclear activity in animal development.
    Biol Rev Camb Philos Soc. 1968 May;43(2):233-67 PMID: 4869950
  11. Characterization and immunochemical localization of a basic protein from pig brain. II. Peptide maps and tissue-specific nuclear localization.
    J Biol Chem. 1968 May 25;243(10):2507-13 PMID: 4967956
  12. Changing patterns of histone acetylation and RNA synthesis in regeneration of the liver.
    Proc Natl Acad Sci U S A. 1968 Apr;59(4):1337-44 PMID: 5240033
  13. Differences in turnover between histones and their acetyl N-terminal groups.
    Biochim Biophys Acta. 1968 Jun 26;160(2):217-23 PMID: 4298007
  14. Acetylation of histones by a kinase from rat liver nucle.
    Biochem Biophys Res Commun. 1968 Jul 26;32(2):117-21 PMID: 4175011
  15. Chemical studies of histone acetylation. The occurrence of epsilon-N-acetyllysine in the f2a1 histone.
    J Biol Chem. 1968 Oct 10;243(19):5018-22 PMID: 5679978
  16. Histone acetylation in scrapie-affected mouse brain.
    Experientia. 1968 Aug 15;24(8):793-4 PMID: 5693209
  17. Hybridizable ribonucleic acid of rat brain.
    Biochem J. 1968 Oct;109(4):533-41 PMID: 5683505
  18. Histone phosphorylation and cell division.
    Biochem J. 1968 Sep;109(3):24P-25P PMID: 5685848
  19. Reactivation of DNA synthesis in mammalian neuron nuclei after fusion with cells of an undifferentiated fibroblast line.
    Exp Cell Res. 1968 Oct;53(1):316-8 PMID: 5749528
  20. Histone phosphorylation: stimulation by adenosine 3',5'-monophosphate.
    Science. 1968 Nov 1;162(3853):579-80 PMID: 4303296
  21. Identification of a prednisolone derivative interacting with calf thymus histones.
    J Pharm Sci. 1968 Dec;57(12):2116-9 PMID: 4974347
  22. Inherited characteristics affecting neurotropic behaviour of scrapie.
    Biochem J. 1969 Feb;111(3):17P PMID: 5767040
  23. Calf and pea histone IV. II. The complete amino acid sequence of calf thymus histone IV; presence of epsilon-N-acetyllysine.
    J Biol Chem. 1969 Jan 25;244(2):319-34 PMID: 5773298
  24. Histone acetylation: cytological evidence in human lymphocytes.
    Exp Cell Res. 1969 Feb;54(2):257-60 PMID: 5776481
  25. The acetylation of histones of rat liver nuclei in vitro by acetyl-CoA.
    Hoppe Seylers Z Physiol Chem. 1969 Feb;350(2):150-4 PMID: 5776500
  26. A quantitative comparison of histones from immature and mature erythroid cells of the duck.
    Biochim Biophys Acta. 1969 Mar;175(2):414-8 PMID: 5778176
  27. DNA synthesis in the glial cells of scrapie-affected mouse brain.
    J Neurochem. 1969 Apr;16(4):543-8 PMID: 5813969
  28. The interaction of RNA polymerase with histones.
    Proc Natl Acad Sci U S A. 1969 Apr;62(4):1218-25 PMID: 5256418
  29. Role of chromatin in estrogen action in the uterus. II. Hormone-induced synthesis of nonhistone acidic proteins which restore histone-inhibited DNA-dependent RNA synthesis.
    Proc Natl Acad Sci U S A. 1969 Jun;63(2):465-72 PMID: 5257138
  30. Extraction of rat myelin basic protein free of other basic proteins of whole central nervous system tissue. An analysis of its electrophoretic heterogeneity.
    J Biol Chem. 1969 Aug 25;244(16):4268-72 PMID: 5806575
  31. The relationship between histone and DNA synthesis in HeLa cells.
    Biochim Biophys Acta. 1969 Aug 20;186(2):349-57 PMID: 5822837
  32. Developmental and regional variations in ribonucleic acid synthesis on cerebral chromatin.
    Biochem J. 1969 Nov;115(2):341-9 PMID: 4907883
  33. Protein measurement with the Folin phenol reagent.
    J Biol Chem. 1951 Nov;193(1):265-75 PMID: 14907713
  34. Separation of acetyl transfer enzymes in pigeon liver extract.
    J Biol Chem. 1952 May;196(1):89-103 PMID: 12980945
  35. Improved fractionations of arginine-rich histones from calf thymus.
    Biochem J. 1960 Dec;77:631-6 PMID: 13790220
  36. The relative ability of reconstituted nucleohistones to allow DNA-dependent RNA synthesis.
    Biochem Biophys Res Commun. 1963 Jul 26;12:175-9 PMID: 13954640
  37. Incorporation of thymidine-H3 by cells in normal and injured mouse spinal cord.
    J Neuropathol Exp Neurol. 1962 Oct;21:597-609 PMID: 14011122
  38. REPRESSED AND ACTIVE CHROMATIN ISOLATED FROM INTERPHASE LYMPHOCYTES.
    Proc Natl Acad Sci U S A. 1963 Dec;50:1026-32 PMID: 14096174
  39. HISTONES: SPECIES AND TISSUE SPECIFICITY.
    Science. 1964 Sep 4;145(3636):1059-61 PMID: 14172621
  40. REGULATION OF CEREBRAL METABOLISM OF AMINO ACIDS-3. CHARACTERISTICS OF AMINO ACID INCORPORATION INTO PROTEIN OF MICROSOMAL AND RIBOSOMAL PREPARATIONS OF RAT CEREBRAL CORTEX.
    J Neurochem. 1964 Aug;11:567-82 PMID: 14206459
  41. A study of the conditions and mechanism of the diphenylamine reaction for the colorimetric estimation of deoxyribonucleic acid.
    Biochem J. 1956 Feb;62(2):315-23 PMID: 13293190
Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1190665
Published
1970-10-00
Pages
665-72
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1179452
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1190665 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2