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PMID: 4120639 Published · ppublish English Journal Article

Isolation and properties of an RNA polymerase from influenza virus-infected cells.

Journal of virology ·Vol. 11 ·No. 3 ·1973-03-00 ·Pages 441-8

Compans RW, Caliguiri LA

Abstract

Structures with RNA polymerase activity were isolated from influenza virus-infected cells, and consisted of ribonucleoprotein (RNP) complexes, similar in morphology to the viral internal component or nucleocapsid. The isolation procedure involved fractionation of infected cells in a discontinuous sucrose gradient, in which enzyme activity was concentrated in a fraction of intermediate density which contains both smooth and rough cytoplasmic membranes. The RNPs with polymerase activity were further purified in a velocity gradient, after which the peak fractions showed a 35-fold purification of the polymerase activity when compared with cytoplasmic extracts. The NP polypeptide, which is the subunit of the virion RNP, was the only virus-specific polypeptide detected in these RNP structures.

MeSH Terms
Animals Carbon Isotopes Cattle Cell Fractionation Cell Line Centrifugation, Density Gradient Cricetinae Cytoplasm/enzymology DNA-Directed RNA Polymerases/analysis,isolation & purification,metabolism Electrophoresis, Polyacrylamide Gel Glucosamine/metabolism Kidney Microscopy, Electron Nucleoproteins/analysis Nucleotides/metabolism Orthomyxoviridae/analysis Peptides/analysis Protein Hydrolysates/metabolism Staining and Labeling Tritium Viral Proteins/analysis
Chemicals
Carbon Isotopes Nucleoproteins Nucleotides Peptides Protein Hydrolysates Viral Proteins Tritium DNA-Directed RNA Polymerases Glucosamine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Compans R W
Caliguiri L A
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31 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.11.3.441-448.1973
Published
1973-03-00
Pages
441-8
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355119
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.11.3.441-448.1973 PMC Full Text View journal details
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