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PMID: 4336942 Published · ppublish English Journal Article

Evidence for the identity of the major apoprotein in low density and very low density lipoproteins in normal subjects and patients with familial hyperlipoproteinemia.

The Journal of clinical investigation ·Vol. 51 ·No. 6 ·1972-06-00 ·Pages 1486-94

Gotto AM, Brown WV, Levy RI, Birnbaumer ME, Fredrickson DS

Abstract

The major apoprotein(s) from human plasma low density lipoproteins was isolated and compared with a major protein fraction (fraction I) from very low density lipoproteins (VLDL). Fraction I had been previously found to comprise approximately 40% of the total protein of VLDL. Fraction I from VLDL and apoLDL from normal subjects were indistinguishable in amino acid compositions and circular dichroic spectra. They yielded indistinguishable displacement curves of LDL-(125)I by radioimmunoassay and formed immunoprecipitin lines of complete identity. Fraction I from VLDL of normal subjects was compared with the fraction isolated from patients with familial types II, III, IV, and V hyperlipoproteinemia. There were no detectable differences between any of these fractions in amino acid compositions, circular dichroic spectra, and immunochemical properties. It was, therefore, concluded that short of peptide mapping or determination of amino acid sequence, fraction I from VLDL of each subject with familial hyperlipoproteinemia appears to be identical with fraction I and apoLDL from normal individuals.A new convenient method of preparation of soluble apoLDL, modified from a procedure previously described from this laboratory, is presented.

MeSH Terms
Amino Acid Sequence Amino Acids/analysis Apoproteins/blood,isolation & purification Chromatography, Gel Circular Dichroism Humans Hyperlipidemias/blood,genetics Immunodiffusion Iodine Isotopes Lipoproteins/blood Lipoproteins, LDL/blood Lipoproteins, VLDL/blood Radioimmunoassay
Chemicals
Amino Acids Apoproteins Iodine Isotopes Lipoproteins Lipoproteins, LDL Lipoproteins, VLDL
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Gotto A M
Brown W V
Levy R I
Birnbaumer M E
Fredrickson D S
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40 references, click to expand
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Article Info
Journal
The Journal of clinical investigation
Abbr.
J Clin Invest
ISSN
0021-9738
DOI
10.1172/JCI106945
Published
1972-06-00
Pages
1486-94
Language
English
Region
United States
NLM ID
7802877
PMCID
PMC292286
Subset
IM
External Links
PubMed source DOI: 10.1172/JCI106945 PMC Full Text View journal details
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