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PMID: 4351176 Published · ppublish English Journal Article

Effect of insulin on the growth pattern and adenylate cyclase activity of BHK fibroblasts.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 5 ·1973-05-00 ·Pages 1388-92

Jiménez de Asúa L, Surian ES, Flawia MM, Torres HN

Abstract

BHK 21/13 fibroblasts grown in the presence of insulin show some characteristics of a transformed strain. Cells broaden, cross each other intricately, and form colonies on both polystyrene dishes and soft agar. The effect of insulin is prevented by addition of N(6)-butyryl adenosine 3':5'-cyclic monophosphate and theophylline to the culture medium. Insulin causes a decrease in the concentration of intracellular adenosine 3':5'-cyclic monophosphate and inhibition of adenylate cyclase activity in BHK fibroblast membranes. This inhibition is observed at insulin concentrations of 0.1 nM or higher.

MeSH Terms
Adenylyl Cyclase Inhibitors Adenylyl Cyclases/analysis Animals Cell Line Cell Membrane/enzymology Cell Transformation, Neoplastic/drug effects Cells, Cultured Cricetinae Culture Media Cyclic AMP/analysis,pharmacology Fibroblasts/cytology,enzymology Insulin/pharmacology Insulin Antagonists Kidney Mitosis/drug effects Theophylline/pharmacology
Chemicals
Adenylyl Cyclase Inhibitors Culture Media Insulin Insulin Antagonists Theophylline Cyclic AMP Adenylyl Cyclases
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Jiménez de Asúa L
Surian E S
Flawia M M
Torres H N
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22 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.5.1388
Published
1973-05-00
Pages
1388-92
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433503
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.5.1388 PMC Full Text View journal details
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