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PMID: 4362639 Published · ppublish English Journal Article

Expression of differentiated functions in hepatoma cell hybrids: high frequency of induction of mouse albumin production in rat hepatoma-mouse lymphoblast hybrids.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 3 ·1974-03-00 ·Pages 927-31

Malawista SE, Weiss MC

Abstract

We have studied the production of serum albumin by somatic hybrids between well-differentiated 2s and 1s rat hepatoma cells (Faza), which produce serum albumin, and sub-diploid mouse leukemic lymphoblasts (Lc), which do not produce albumin. We determined the rat or mouse origin of the albumin by double immunodiffusion, using immuno-adsorbed noncrossreacting antisera. Each of 12 karyologically identified 2s hybrid clones (Lc2F) produces both rat and mouse albumin. Moreover, unlike 1s hybrids reported previously, eight of nine 1s hybrids (LcF) also produce mouse albumin; six of them produce rat albumin as well. One clone from the 1s cross produces only rat albumin.

MeSH Terms
Albumins/analysis,biosynthesis Animals Carcinoma, Hepatocellular Cell Fusion Cell Line Chromosomes Clone Cells Cross Reactions Hybrid Cells/metabolism Immunoassay Immunodiffusion Karyotyping Leukemia, Lymphoid Liver Neoplasms Mice Parainfluenza Virus 1, Human Rabbits/immunology Rats Serum Albumin/biosynthesis Species Specificity
Chemicals
Albumins Serum Albumin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Malawista S E
Weiss M C
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21 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.71.3.927
Published
1974-03-00
Pages
927-31
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC388130
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.71.3.927 PMC Full Text View journal details
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