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PMID: 4377220 Published · ppublish English Journal Article

Lipid and basic protein interaction of myelin.

The Biochemical journal ·Vol. 143 ·No. 1 ·1974-10-00 ·Pages 39-45

Banik NL, Davison AN

Abstract

1. Purified myelin labelled with [(3)H]myo-inositol or [1-(14)C]acetate was incubated with trypsin or acetylated trypsin at 37 degrees C, pH8.0 for 30min. 2. After incubation and centrifugation analysis of the myelin pellet showed marked digestion of basic protein on polyacrylamide-gel electrophoresis. Proteolipid and Wolfgram proteins remained unchanged. 3. A loss of 15% of total protein and loss of all classes of lipids was also found. Most significant lipid losses were phosphoinositides, phosphatidylserine and sulphatide. 4. A low-density material containing more phospholipid than cholesterol and galactolipid was isolated from the supernatant obtained after centrifugation of trypsin-treated myelin. 5. Interaction of sulphatide and myelin basic protein was shown to take place in a biphasic system. Basic protein does not form any complex either with cerebroside or cholesterol in the same solvent system. 6. The release of acidic lipids from myelin suggests that they may be linked to basic protein by ionic forces and the neutral lipids may be by lipid-lipid interactions. 7. The relevance of these studies as a model of brain degeneration is discussed.

MeSH Terms
Animals Brain Chemistry Cell Membrane Female Lipoproteins/analysis,isolation & purification Male Microscopy, Electron Models, Molecular Myelin Sheath Nerve Tissue Proteins Phosphatidylinositols Rats Scintillation Counting Structure-Activity Relationship Trypsin/metabolism
Chemicals
Lipoproteins Nerve Tissue Proteins Phosphatidylinositols Trypsin
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Banik N L
Davison A N
References (48)
48 references, click to expand
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Article Info
Journal
The Biochemical journal
Abbr.
Biochem J
ISSN
0264-6021
DOI
10.1042/bj1430039
Published
1974-10-00
Pages
39-45
Language
English
Region
England
NLM ID
2984726R
PMCID
PMC1168349
Subset
IM
External Links
PubMed source DOI: 10.1042/bj1430039 PMC Full Text View journal details
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