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PMID: 4504353 Published · ppublish English Journal Article

Inhibition of influenza virus replication by -amanitin: mode of action.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 6 ·1972-06-00 ·Pages 1421-4

Mahy BW, Hastie ND, Armstrong SJ

Abstract

The replication of influenza virus in chick embryo fibroblast cells is inhibited by alpha-amanitin added during the first 2 hr of infection at concentrations similar to those required to inhibit cellular DNA-dependent RNA polymerase form II in vivo. Of two periods of increased RNA synthesis observed in cells infected with influenza virus, only the first, occurring from 0 to 2 hr after infection, is sensitive to alpha-amanitin. During this early period, there is a stimulation of the activity of DNA-dependent RNA polymerase II of nuclei isolated from infected cells. The data suggest that DNA transcription mediated by polymerase II is essential for influenza virus replication.

MeSH Terms
Animals Antiviral Agents/pharmacology Cells, Cultured/enzymology,metabolism Chick Embryo DNA/metabolism Depression, Chemical Fibroblasts/enzymology,metabolism Mycotoxins/pharmacology Orthomyxoviridae/metabolism Peptides/pharmacology RNA/biosynthesis RNA Nucleotidyltransferases/metabolism Virus Replication/drug effects
Chemicals
Antiviral Agents Mycotoxins Peptides RNA DNA RNA Nucleotidyltransferases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mahy B W
Hastie N D
Armstrong S J
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28 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.6.1421
Published
1972-06-00
Pages
1421-4
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426716
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.6.1421 PMC Full Text View journal details
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