Germ line basis for antibody diversity: immunoglobulin VH-and CH-gene frequencies measured by DNA-RNA hybridization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 71 ·No. 1 ·1974-01-00 ·Pages 99-103

Premkumar E, Shoyab M, Williamson AR

Abstract

The reiteration frequency for mouse immunoglobulin V(H)-genes and C(H)-genes has been directly estimated by hybridization of purified MOPC 315 alpha-chain mRNA with a vast excess of mouse DNA. A biphasic C(o)t curve resulted. The low C(o)t transition (C(o)t(1/2) about 1.5) was interpreted as hybridization to V(H)-genes and the high C(o)t transition (C(o)t(1/2) about 10(3)) as hybridization to C(H)-genes. These values correspond to about 5000 V(H)-genes and less than 8 C(H)-genes. This germ-line content of V(H)-genes is sufficient to account for antibody diversity given a comparable set of V(L)-genes. Minimal-gene models are invalidated and there is no need to invoke somatic generators of diversity.

MeSH Terms

Animals Cell Line Centrifugation, Density Gradient Cytidine/metabolism DNA/isolation & purification,metabolism Gene Frequency Genes Hot Temperature Immunoglobulin Fragments Immunoglobulins/biosynthesis Kinetics Mice Multiple Myeloma Myeloma Proteins/biosynthesis Nucleic Acid Denaturation Nucleic Acid Hybridization RNA, Messenger/biosynthesis,isolation & purification,metabolism Tritium Uridine/metabolism

Chemicals

Immunoglobulin Fragments Immunoglobulins Myeloma Proteins RNA, Messenger Tritium Cytidine DNA Uridine

Authors & Affiliations

3 authors, click to expand affiliations / ORCID

Premkumar E

Shoyab M

Williamson A R

References (17)

17 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.71.1.99

Published

1974-01-00

Pages

99-103

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC387941

Subset

External Links

PubMed source DOI: 10.1073/pnas.71.1.99 PMC Full Text View journal details

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Germ line basis for antibody diversity: immunoglobulin VH-and CH-gene frequencies measured by DNA-RNA hybridization.

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