Home LiteratureArticle Details
PMID: 4509648 Published · ppublish English Journal Article

Estimation of light-chain gene reiteration of mouse immunoglobulin by DNA-RNA hybridization.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 173-8

Delovitch TL, Baglioni C

Abstract

A 12S species of RNA has been isolated from membrane-bound polyribosomes of MPC-11 mouse myeloma cells. This 12S RNA is translated by an extract of Krebs-II mouse ascites cells into immunoglobulin light chain. Labeled 12S RNA has been prepared by incubation of myeloma cells with [(3)H]uridine in the presence of low concentrations of actinomycin D and ethidium bromide. This RNA has been hybridized under conditions of DNA excess to mouse myeloma DNA and to liver DNA. A C(0)t(1/2) of about 150 has been obtained, corresponding to a reiteration frequency of about 40. Unlabeled 12S RNA competes with the labeled species in hybridization experiments, whereas globin mRNA or mouse ascites 12S RNA does not. It is suggested that 12S RNA hybridizes only to V(kappa)-genes of the same subclass, and that there may be several hundred genes coding for V(kappa)-regions of all subclasses in a mouse genome. Moreover, gene amplification in myeloma cells is not detected.

MeSH Terms
Animals Carbon Isotopes Cell Line Cell-Free System Centrifugation, Density Gradient DNA, Neoplasm/metabolism Electrophoresis, Polyacrylamide Gel Goats/immunology HeLa Cells Immunoglobulin Fragments/isolation & purification Immunoglobulins/analysis,biosynthesis Mice Multiple Myeloma Myeloma Proteins/biosynthesis Neoplasms, Experimental Nucleic Acid Hybridization Precipitin Tests Protein Biosynthesis RNA, Messenger/isolation & purification,metabolism RNA, Ribosomal/isolation & purification,metabolism Rabbits/immunology Sulfur Isotopes Tritium
Chemicals
Carbon Isotopes DNA, Neoplasm Immunoglobulin Fragments Immunoglobulins Myeloma Proteins RNA, Messenger RNA, Ribosomal Sulfur Isotopes Tritium
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Delovitch T L
Baglioni C
References (38)
38 references, click to expand
  1. Assembly of membrane-bound polyribosomes.
    Nat New Biol. 1971 Jul 7;232(27):8-12 PMID: 5284428
  2. Properties of halves of immunoglobulin light chains.
    Proc Natl Acad Sci U S A. 1969 Dec;64(4):1257-63 PMID: 4195396
  3. Messenger RNA for globin in the postribosomal supernatant of rabbit reticulocytes.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1425-8 PMID: 4504354
  4. Small scale preparative gel electrophoresis of ribonucleic acids.
    Biochim Biophys Acta. 1972 Aug 25;277(2):301-5 PMID: 5075288
  5. The specificity of molecular hybridization reactions.
    Annu Rev Biochem. 1970;39:131-50 PMID: 4922848
  6. Translation of reovirus messenger RNAs synthetesized in vitro into reovirus polypeptides by several mammalian cell-free extracts.
    Proc Natl Acad Sci U S A. 1972 Sep;69(9):2649-53 PMID: 4506788
  7. Mechanism of antibody diversity: germ line basis for variability.
    Science. 1970 Apr 17;168(3929):325-34 PMID: 4190985
  8. Somatic translocation of antibody genes.
    Nature. 1970 Jul 25;227(5256):341-8 PMID: 4193641
  9. Isolation of messenger-like RNA from immunochemically separated polyribosomes.
    J Mol Biol. 1972 Aug 28;69(3):373-86 PMID: 5080440
  10. Selective inhibition of the synthesis of mitochondria-associated RNA by ethidium bromide.
    J Mol Biol. 1969 Aug 28;44(1):195-204 PMID: 5811827
  11. Immunoglobulin structure: amino terminal sequences of kappa chains from genetically similar mice (BALB/c).
    Science. 1970 Dec 11;170(3963):1207-10 PMID: 4097844
  12. In vitro synthesis of DNA complementary to rabbit reticulocyte 10S RNA.
    Nat New Biol. 1972 Feb 9;235(58):163-7 PMID: 4334193
  13. Purification and properties of biologically active messenger RNA for a myeloma light chain.
    Proc Natl Acad Sci U S A. 1972 Jul;69(7):1967-71 PMID: 4505674
  14. Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.
    Proc Natl Acad Sci U S A. 1972 May;69(5):1299-303 PMID: 4338590
  15. SEDIMENTATION STUDIES OF THE SIZE AND SHAPE OF DNA.
    J Mol Biol. 1965 Feb;11:373-90 PMID: 14290352
  16. Reiteration frequency of haemoglobin genes in the duck.
    Nat New Biol. 1972 Feb 23;235(60):231-4 PMID: 4502410
  17. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms.
    Science. 1968 Aug 9;161(3841):529-40 PMID: 4874239
  18. Translation of messenger RNA for immunoglobulin light chains in a cell-free system from Krebs II ascites cells.
    FEBS Lett. 1972 Jun 15;23(2):244-8 PMID: 4673707
  19. Use of low concentrations of actinomycin D in the study of RNA synthesis in Ehrlich ascites cells.
    J Mol Biol. 1966 Sep;20(1):63-73 PMID: 6008160
  20. An analysis of the sequences of the variable regions of Bence Jones proteins and myeloma light chains and their implications for antibody complementarity.
    J Exp Med. 1970 Aug 1;132(2):211-50 PMID: 5508247
  21. In vitro synthesis of DNA components of human genes for globins.
    Nat New Biol. 1972 Feb 9;235(58):167-9 PMID: 4334194
  22. Translation of messenger RNA for histones from HeLa cells by a cell-free extract from mouse ascites tumor.
    Proc Natl Acad Sci U S A. 1972 Aug;69(8):2095-9 PMID: 4506079
  23. Bence-Jones proteins and light chains of immunoglobulins. I. Formation and characterization of amino-terminal (variant) and carboxyl-terminal (constant) halves.
    J Biol Chem. 1969 Jun 25;244(12):3393-404 PMID: 4183096
  24. Quantitation of immunoglobulin genes by nucleic acid hybridization with RNA from myeloma and spleen microsomes.
    J Immunol. 1972 Mar;108(3):755-64 PMID: 5011755
  25. Specific IgG mRNA molecules from myeloma cells in heterogeneous nuclear and cytoplasmic RNA containing poly-A.
    Nature. 1972 Sep 15;239(5368):143-6 PMID: 4561958
  26. Ribonucleoprotein particles involved in HeLa mitochondrial protein synthesis.
    Nat New Biol. 1971 Feb 3;229(5):136-9 PMID: 5280092
  27. Mammalian cell-free protein synthesis directed by viral ribonucleic acid.
    Eur J Biochem. 1970 Dec;17(2):328-38 PMID: 5500401
  28. Synthesis of a mouse immunoglobulin light chain in a rabbit reticulocyte cell-free system.
    Nat New Biol. 1971 Apr 7;230(14):172-6 PMID: 4101841
  29. Synthesis, assembly, and secretion of gamma globulin by mouse myeloma cells. I. Adaptation of the Merwin plasma cell tumor-11 to culture, cloning, and characterization of gamma globulin subunits.
    J Exp Med. 1970 Mar 1;131(3):515-41 PMID: 4189836
  30. A study of immunoglobulin structure. I. The fingerprinting of aminoethylated Bence-Jones proteins.
    Biochim Biophys Acta. 1965 Dec 16;111(2):479-84 PMID: 5879480
  31. Evidence for single copies of globin genes in the mouse genome.
    Nature. 1972 Sep 22;239(5369):219-21 PMID: 4117010
  32. In vitro synthesis of DNA complementary to purified rabbit globin mRNA (RNA-dependent DNA polymerase-reticulocyte-hemoglobin-density gradient centrifugation-oligo(dT) primer).
    Proc Natl Acad Sci U S A. 1972 Jan;69(1):264-8 PMID: 4333043
  33. Acrylamide gel electrophoresis of HeLa cell nucleolar RNA.
    Proc Natl Acad Sci U S A. 1967 Sep;58(3):1088-95 PMID: 5233837
  34. DNA-RNA hybridization in vast DNA excess.
    Nat New Biol. 1971 May 5;231(18):8-12 PMID: 4932893
  35. AMINO ACID ACCEPTOR ACTIVITY OF ENZYMICALLY ALTERED SOLUBLE RNA FROM ESCHERICHIA COLI.
    Biochim Biophys Acta. 1964 Apr 27;80:574-86 PMID: 14156730
  36. Rate of somatic mutation in immunoglobulin production by mouse myeloma cells.
    Proc Natl Acad Sci U S A. 1971 Jan;68(1):219-23 PMID: 5276293
  37. Reiteration and clustering of DNA sequences complementary to histone messenger RNA.
    Nat New Biol. 1971 Apr 7;230(14):165-9 PMID: 5279989
  38. THE CELLULAR SITES OF SYNTHESIS OF RIBOSOMAL AND 4S RNA.
    Proc Natl Acad Sci U S A. 1962 Dec;48(12):2179-86 PMID: 16591032
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.1.173
Published
1973-01-00
Pages
173-8
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433209
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.1.173 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2