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PMID: 4338590 Published · ppublish English Journal Article

Increased efficiency of exogenous messenger RNA translation in a Krebs ascites cell lysate.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 69 ·No. 5 ·1972-05-00 ·Pages 1299-303

Metafora S, Terada M, Dow LW, Marks PA, Bank A

Abstract

Addition of a 0.5 M KCl wash fraction from rabbit reticulocyte ribosomes causes a 3- to 10-fold increase in the extent of translation of natural mRNAs by Krebs-cell lysates. In the presence of the wash fraction, 1 pmol of rabbit or mouse 10S RNA directs the incorporation of 80 pmol of leucine into rabbit globin. The addition of human 10S RNA results in the synthesis of equal amounts of human alpha and beta chains, identified by column chromatography. The stimulation by the wash fraction is almost completely dependent on added mammalian tRNA. In contrast to the wash fraction from rabbit reticulocytes, the wash fraction isolated from Krebs-cell ribosomes is inhibitory to both endogenous and exogenous mRNA translation. The stimulation by the wash fraction from rabbit ribosomes is not specific for globin mRNAs, but also increases endogenous, phage Qbeta, and viral RNA-directed protein synthesis.

MeSH Terms
Amino Acyl-tRNA Synthetases/metabolism Animals Carcinoma, Krebs 2 Cell-Free System Cells, Cultured Centrifugation, Density Gradient Encephalomyocarditis virus/metabolism Globins/biosynthesis Humans Leucine/metabolism Mice Mice, Inbred Strains Peptide Chain Initiation, Translational RNA, Messenger/metabolism RNA, Neoplasm/metabolism RNA, Transfer/metabolism RNA, Viral/metabolism Rabbits Reticulocytes/metabolism Ribosomes/enzymology Tritium
Chemicals
RNA, Messenger RNA, Neoplasm RNA, Viral Tritium Globins RNA, Transfer Amino Acyl-tRNA Synthetases Leucine
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Metafora S
Terada M
Dow L W
Marks P A
Bank A
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20 references, click to expand
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Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.69.5.1299
Published
1972-05-00
Pages
1299-303
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC426686
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.69.5.1299 PMC Full Text View journal details
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