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PMID: 455411 Published · ppublish English Journal Article

Effects of caffeine and D2O on persistence and de novo generation of intrinsic oscillatory contraction automaticity in Physarum.

Cell and tissue research ·Vol. 197 ·No. 3 ·1979-04-12 ·Pages 479-99

Götz von Olenhusen KG, Wohlfarth-Bottermann KE

Abstract

The present investigation was performed in an attempt to contribute to answering the question whether the plasmalemma of the plasmodial stage of Physarum represents the site of a trigger mechanism for the oscillating contraction activity of cytoplasmic actomyosin. The effects of the following substances on persistence of tensiometrically measured longitudinal and radial activities of Physarum veins and on de novo generation of activities in experimentally generated drops were studied: caffeine, theophylline, acetylcholinium chloride, procaine, physostigminium salicylate, iso-ompa, nifedipin, sodium nitroprusside, potassium thiocyanate, D2O; as well as the effects of ions such as La+++ and high outer concentrations of Na+ and K+. Some of the substances were applied simultaneously for comparison externally (by bathing solutions) and internally (by injection). The experimental data speak against the existence of electrogenic rhythmical Ca++, Na+ or K+ pumps across the plasmalemma which could have a triggering function for the oscillation. The contraction activities of the cytoplasmic actomyosin seem to represent a spontaneous endogeneous oscillation which can be modulated via the plasmalemma during chemotaxis.

MeSH Terms
Biological Transport, Active Caffeine/pharmacology Cell Membrane/metabolism,physiology Deuterium/pharmacology Dose-Response Relationship, Drug Movement/drug effects Physarum/metabolism,physiology Theophylline/pharmacology
Chemicals
Caffeine Deuterium Theophylline
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Götz von Olenhusen K G
Wohlfarth-Bottermann K E
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41 references, click to expand
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Article Info
Journal
Cell and tissue research
Abbr.
Cell Tissue Res
ISSN
0302-766X
DOI
10.1007/BF00233572
Published
1979-04-12
Pages
479-99
Language
English
Region
Germany
NLM ID
0417625
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00233572 View journal details
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