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PMID: 4560343 Published · ppublish English Journal Article

Surface antigens of immunocompetent cells. 3. In vitro studies of the role of B and T cells in immunological memory.

The Journal of experimental medicine ·Vol. 136 ·No. 4 ·1972-10-01 ·Pages 663-75

Mond JJ, Takahashi T, Thorbecke GJ

Abstract

The effect of preincubation with anti-theta or anti-mouse immunoglobulin (Ig) and complement (C') on immune responsiveness of spleen cells from BALB/c mice immunized with sheep erythrocytes (SE) was investigated. Both treatments greatly depressed the remaining ability to produce a secondary response to SE in vitro. Normal BALB/c spleen cells were far less effective in reconstituting the responses of such depleted cell populations than were much smaller numbers of untreated immune spleen cells. Thymus-derived cell (T cell) memory appeared early after immunization and showed specificity for the immunizing antigens. Recombination of anti-Ig-treated with anti-theta-treated immune spleen cells resulted in virtually complete reconstitution of responsiveness. The presence of immunological memory in T cells and the nature of their surface receptors are discussed.

MeSH Terms
Animals Antibody Formation Antibody-Producing Cells Antigens Bone Marrow/immunology Bone Marrow Cells Cell Membrane/immunology Cells, Cultured Complement System Proteins Erythrocytes/immunology Hemolytic Plaque Technique Immune Sera Immunity, Maternally-Acquired Immunoglobulin G Immunologic Memory Immunosuppression Therapy Leukemia, Experimental/immunology Lymphocytes/immunology Male Mice Sheep/immunology Spleen/cytology,immunology Thymus Gland/cytology,immunology
Chemicals
Antigens Immune Sera Immunoglobulin G Complement System Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Mond J J
Takahashi T
Thorbecke G J
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44 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.136.4.663
Published
1972-10-01
Pages
663-75
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2139273
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.136.4.663 PMC Full Text View journal details
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