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PMID: 4567332 Published · ppublish English Journal Article

Colicin E3: a unique endoribonuclease.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 70 ·No. 1 ·1973-01-00 ·Pages 156-60

Meyhack B, Meyhack I, Apirion D

Abstract

The question of whether or not a cellular ribonuclease is involved in the cleavage of 16S ribosomal RNA by colicin E3 was investigated. For this purpose ribosomes from strains devoid of some ribonucleases or ribosomes in which ribonucleases had been inactivated by heat or removed by extensive washings were used for the colicin reaction. Since the 16S RNA of all these different ribosomes, and even of the most extensively washed ribosomes, was cleaved by colicin E3, it is suggested that cellular ribonucleases are not involved in colicin E3 action. Thus, colicin E3 seems to be a unique endoribonuclease.

MeSH Terms
Colicins/metabolism Electrophoresis, Polyacrylamide Gel Endonucleases/metabolism Escherichia coli/enzymology,metabolism Mutation Phosphorus Isotopes Polyribonucleotide Nucleotidyltransferase/metabolism Proteins/isolation & purification,metabolism RNA, Ribosomal/isolation & purification,metabolism Ribonucleases/metabolism Ribosomes/analysis,metabolism Temperature
Chemicals
Colicins Phosphorus Isotopes Proteins RNA, Ribosomal Polyribonucleotide Nucleotidyltransferase Endonucleases Ribonucleases
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Meyhack B
Meyhack I
Apirion D
References (24)
24 references, click to expand
  1. Isolation and characterization of ribonuclease I mutants of Escherichia coli.
    J Mol Biol. 1966 Mar;16(1):67-84 PMID: 5331244
  2. The sedimentation behaviour of ribonuclease-active and -inactive ribosomes from bacteria.
    Biochem J. 1965 Sep;96(3):671-80 PMID: 5324303
  3. Inactivation of ribosomes by colicin E3 in vitro: Requirement for 50 S ribosomal subunits.
    FEBS Lett. 1972 Apr 15;22(1):73-75 PMID: 11946564
  4. Specific inactivation of ribosomes by colicin E3 in vitro and mechanism of immunity in colicinogenic cells.
    Nat New Biol. 1971 Dec 1;234(48):133-7 PMID: 4332178
  5. Specific inactivation of 16S ribosomal RNA induced by colicin E3 in vivo.
    Proc Natl Acad Sci U S A. 1971 May;68(5):964-8 PMID: 4930244
  6. DISC ELECTROPHORESIS IN POLYACRYLAMIDE GELS: EXTENSION TO NEW CONDITIONS OF PH AND BUFFER.
    Ann N Y Acad Sci. 1964 Dec 28;121:373-81 PMID: 14240536
  7. FRACTIONATION OF THE RIBOSOMAL PROTEIN OF ESCHERICHIA COLI BY ION-EXCHANGE CHROMATOGRAPHY ON CARBOXYMETHYL CELLULOSE.
    Biochim Biophys Acta. 1964 Apr 27;80:594-600 PMID: 14156731
  8. Purification and properties of a potassium-activated phosphodiesterase (RNAase II) from Escherichia coli.
    Biochemistry. 1965 Jul;4(7):1319-30 PMID: 5323137
  9. Purification and characterization of colicin E2 and colicin E3.
    J Biol Chem. 1967 Nov 25;242(22):5360-8 PMID: 4965137
  10. Effect of colicin E3 upon the 30S ribosomal subunit of Escherichia coli.
    Proc Natl Acad Sci U S A. 1971 May;68(5):959-63 PMID: 4930243
  11. Inactivation of ribosomes in vitro by colicin E 3 and its mechanism of action.
    Proc Natl Acad Sci U S A. 1972 Mar;69(3):549-52 PMID: 4551976
  12. Inactivation of ribosomes in vitro by colicin E 3 .
    Proc Natl Acad Sci U S A. 1971 Oct;68(10):2421-5 PMID: 4944624
  13. The ribosomal proteins of Escherichia coli. I. Purification of the 30S ribosomal proteins.
    Biochemistry. 1969 Jul;8(7):2897-905 PMID: 4897206
  14. Escherichia coli strains with thermolabile ribonuclease II activity.
    J Biol Chem. 1972 Sep 10;247(17):5404-8 PMID: 4560197
  15. Bacterial ribosome.
    Bacteriol Rev. 1970 Sep;34(3):228-77 PMID: 16350216
  16. Purification and properties of ribonuclease III from Escherichia coli.
    J Biol Chem. 1968 Jan 10;243(1):82-91 PMID: 4865702
  17. Colicins and related bacteriocins.
    Annu Rev Microbiol. 1967;21:257-84 PMID: 4860260
  18. Isolation and mapping of polynucleotide phosphorylase mutants of Escherichia coli.
    J Bacteriol. 1969 Mar;97(3):1431-6 PMID: 4887519
  19. Ribosomal proteins. Isolation of the proteins from 30S ribosomal subunits of Escherichia coli.
    Eur J Biochem. 1971 Nov 11;23(1):7-11 PMID: 4942549
  20. Kinetic analysis of the phosphorolysis of oligonucleotides by polynucleotide phosphorylase.
    J Biol Chem. 1970 Mar 10;245(5):995-1004 PMID: 4313707
  21. Altered ribosomes in a suppressor strain of Escherichia coli.
    J Mol Biol. 1966 Apr;16(2):285-301 PMID: 5333511
  22. DISC ELECTROPHORESIS. I. BACKGROUND AND THEORY.
    Ann N Y Acad Sci. 1964 Dec 28;121:321-49 PMID: 14240533
  23. A simple apparatus for vertical flat-sheet polyacrylamide gel electrophoresis.
    Anal Biochem. 1968 Mar;22(3):374-81 PMID: 5672484
  24. Is RNase V a manifestation of RNase II?
    Biochem Biophys Res Commun. 1971 Aug 20;44(4):844-51 PMID: 4942118
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.70.1.156
Published
1973-01-00
Pages
156-60
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC433205
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.70.1.156 PMC Full Text View journal details
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