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PMID: 4590017 Published · ppublish English Journal Article

Replication of bacteriophage phiX174 DNA in a temperature-sensitive dnaC mutant of escherichia coli C.

Journal of virology ·Vol. 13 ·No. 1 ·1974-01-00 ·Pages 146-54

Kranias EG, Dumas LB

Abstract

Bacteriophage phiX174 cannot grow in a temperature-sensitive dnaC mutant of Escherichia coli C at the nonpermissive temperature. The inability to grow is the result of inhibition of virus DNA synthesis. Parental replicative form synthesis is not temperature sensitive. Single-stranded virus DNA continues to be synthesized for at least 45 min after shifting to the nonpermissive temperature late in infection. In contrast, the replication of the replicative form terminates within 5 min after shifting to the nonpermissive temperature.

MeSH Terms
Centrifugation, Density Gradient Coliphages/growth & development,metabolism DNA Replication DNA, Bacterial/biosynthesis DNA, Circular/biosynthesis DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Escherichia coli/metabolism Mutation Phosphorus Radioisotopes Pronase Temperature Thymidine/metabolism Thymine/metabolism Time Factors Tritium Virus Replication
Chemicals
DNA, Bacterial DNA, Circular DNA, Single-Stranded DNA, Viral Phosphorus Radioisotopes Tritium Pronase Thymine Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Kranias E G
Dumas L B
References (27)
27 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.13.1.146-154.1974
Published
1974-01-00
Pages
146-54
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355269
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.13.1.146-154.1974 PMC Full Text View journal details
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