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PMID: 4610175 Published · ppublish English Journal Article

Mechanism of replication of phi x174 single-stranded DNA. IX. Requirement for the Escherichia coli dnaG protein.

Journal of virology ·Vol. 14 ·No. 5 ·1974-11-00 ·Pages 1070-5

McFadden G, Denhardt DT

Abstract

Escherichia coli NY73, possessing a temperature-sensitive mutation in the dnaG locus, was rendered sensitive to bacteriophage phiX174 by P1 transduction. phiX174 reproduces in this strain at 30 C but not at 40 C. All three stages of phiX174 replication, parental replicative form (RF) synthesis, RF replication, and progeny single-stranded DNA synthesis, are thermolabile in this mutant. Competition-annealing data show that both plus- and minus-strand synthesis are equally inhibited after shift up to 40 C during RF replication. We conclude that the dnaG gene product is required for the synthesis of both strands of phiX RF during RF replication and of the complementary strand and viral progeny strands during stages I and III, respectively.

MeSH Terms
Bacterial Proteins/metabolism Centrifugation, Density Gradient Coliphages/growth & development,metabolism DNA Replication DNA Viruses DNA, Bacterial/biosynthesis DNA, Single-Stranded/biosynthesis DNA, Viral/biosynthesis Escherichia coli/metabolism Mutation Nucleic Acid Hybridization Temperature Thymidine/metabolism Transduction, Genetic Tritium Virus Replication
Chemicals
Bacterial Proteins DNA, Bacterial DNA, Single-Stranded DNA, Viral Tritium Thymidine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
McFadden G
Denhardt D T
References (30)
30 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
DOI
10.1128/JVI.14.5.1070-1075.1974
Published
1974-11-00
Pages
1070-5
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC355621
Subset
IM
External Links
PubMed source DOI: 10.1128/JVI.14.5.1070-1075.1974 PMC Full Text View journal details
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