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PMID: 461182 Published · ppublish English Journal Article

DNA chain length markers and the influence of base composition on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels.

Nucleic acids research ·Vol. 6 ·No. 6 ·1979-00-00 ·Pages 2069-87

Frank R, Köster H

Abstract

The specific influence of the four nucleobases on electrophoretic mobility of oligodeoxyribonucleotides in polyacrylamide-gels under denaturing and nondenaturing conditions has been investigated using homooligomers from the four deoxyribonucleotides as chain length standards. Homooligomers of same chain lengths exhibit remarkable differences in mobility. Specific retardation of any other oligonucleotide investigated was found to be mainly dependent on base composition but not on sequence. A simple procedure is presented for calculating mobilities relative to the standards on denaturing gels. This allows a reliable identification of oligonucleotides on acrylamide-gels by exact chain length determination with respect to base composition and furthermore a detailed interpretation of complex reaction mixtures. The homooligomers also show the same differences in mobility on nondenaturing gels. The significance of this effect for strand separation is discussed.

MeSH Terms
Base Sequence DNA Electrophoresis, Polyacrylamide Gel Molecular Weight Oligodeoxyribonucleotides Oligonucleotides Structure-Activity Relationship
Chemicals
Oligodeoxyribonucleotides Oligonucleotides DNA
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Frank R
Köster H
References (17)
17 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/6.6.2069
Published
1979-00-00
Pages
2069-87
Language
English
Region
England
NLM ID
0411011
PMCID
PMC327837
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/6.6.2069 PMC Full Text View journal details
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