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PMID: 4942914 Published · ppublish English Journal Article

Transcriptional organization of the ribosomal RNA cistrons in Escherichia coli.

Doolittle WF, Pace NR

Abstract

The data presented support the hypothesis that 16S, 23S, and 5S ribosomal RNAs of Escherichia coli are transcribed in vivo from transcriptional units consisting of single cistrons for these species arranged in the order 16S-23S-5S, with transcription beginning at the 16S end.

MeSH Terms
Autoradiography Electrophoresis Escherichia coli Genes Genetic Code Models, Biological Nucleotides/analysis Phosphorus Isotopes RNA Nucleotidyltransferases/pharmacology RNA, Bacterial/analysis,biosynthesis RNA, Ribosomal/analysis,biosynthesis Rifampin/pharmacology Tritium Uridine/metabolism
Chemicals
Nucleotides Phosphorus Isotopes RNA, Bacterial RNA, Ribosomal Tritium RNA Nucleotidyltransferases Rifampin Uridine
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Doolittle W F
Pace N R
References (18)
18 references, click to expand
  1. Purification and properties of the replicative intermediate of the RNA bacteriophage R17.
    Proc Natl Acad Sci U S A. 1966 Jun;55(6):1504-11 PMID: 5227669
  2. Relative transcription activity of different segments of the genome throughout the cell division cycle of Escherichia coli. The mapping of ribosomal and transfer RNA and the determination of the direction of replication.
    J Mol Biol. 1967 May 28;26(1):91-105 PMID: 5341414
  3. The kinetics of product appearance and template involvement in the in vitro replication of viral RNA.
    Proc Natl Acad Sci U S A. 1967 Aug;58(2):711-8 PMID: 5233468
  4. Action of rifamycins on RNA polymerase.
    Biochim Biophys Acta. 1968 Mar 18;157(1):215-7 PMID: 4171079
  5. Mode of action of rafamycin on the RNA polymerase reaction.
    Biochim Biophys Acta. 1968 Mar 18;157(1):218-9 PMID: 4868249
  6. Chromosomal location of DNA base sequences complementary to transfer RNA and to 5 s, 16 s and 23 s ribosomal RNA in Bacillus subtilis.
    J Mol Biol. 1968 Apr 14;33(1):123-40 PMID: 4967203
  7. Mutants of Escherichia coli permeable to actinomycin.
    Proc Natl Acad Sci U S A. 1967 Dec;58(6):2315-20 PMID: 4173585
  8. Characterization of a new low molecular weight RNA in HeLa cell ribosomes.
    J Mol Biol. 1968 May 14;33(3):609-23 PMID: 5700415
  9. Ribosomal RNA genes in bacteria: evidence for the nature of the physical linkage between 16S and 23S RNA genes in Bacillus subtilis.
    Proc Natl Acad Sci U S A. 1969 Oct;64(2):642-9 PMID: 4982359
  10. Scattered arrangement of the bacterial ribosomal cistrons.
    Nature. 1970 Jul 18;227(5255):239-42 PMID: 4913638
  11. Synthesis of 5S ribosomal RNA in Escherichia coli after rifampicin treatment.
    Nature. 1970 Oct 10;228(5267):125-9 PMID: 4918254
  12. Location of the genes for 16S and 23S ribosomal RNA in the genetic map of Escherichia coli.
    Proc Natl Acad Sci U S A. 1970 Sep;67(1):26-31 PMID: 4918356
  13. Chain termination of ribosomal RNA synthesis in vitro.
    Nature. 1970 Oct 17;228(5268):235-9 PMID: 4920919
  14. Transcription of the ribosomal RNA genes of an amphibian by the RNA polymerase of a bacterium.
    J Mol Biol. 1970 Jul 28;51(2):361-77 PMID: 4922203
  15. Gene dosage for 5S ribosomal ribonucleic acid in Escherichia coli ad Bacillus megaterium.
    J Bacteriol. 1971 Jan;105(1):142-9 PMID: 4993318
  16. Physical linkage between 5 s, 16 s and 23 s ribosomal RNA genes in Bacillus subtilis.
    J Mol Biol. 1971 Feb 28;56(1):117-27 PMID: 4995655
  17. The ribosomal RNA cistrons.
    Prog Nucleic Acid Res Mol Biol. 1971;11:351-89 PMID: 5004308
  18. [The biosynthesis of beta-galactosidase (lactase) in Escherichia coli; the specificity of induction].
    Biochim Biophys Acta. 1951 Nov;7(4):585-99 PMID: 14904456
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
Published
1971-08-00
Pages
1786-90
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC389293
Subset
IM
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