Isolation of temperature-sensitive mutants of L-cells.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 66 ·No. 2 ·1970-06-00 ·Pages 377-84

Thompson LH, Mankovitz R, Baker RM, Till JE, Siminovitch L, Whitmore GF

Abstract

Procedures are described for the isolation of conditional lethal mutants of mouse L-60T cells. The mutant lines were temperature sensitive by the following criteria: (a) colony-forming ability, (b) growth in suspension culture, and (c) rate of uptake of tritiated-thymidine.

MeSH Terms

Animals Clone Cells/growth & development Culture Techniques L Cells/growth & development,metabolism Lysine/metabolism Mice Mutation Temperature Thymidine/metabolism Tritium Uridine/metabolism

Chemicals

Tritium Lysine Thymidine Uridine

Authors & Affiliations

6 authors, click to expand affiliations / ORCID

Thompson L H

Mankovitz R

Baker R M

Till J E

Siminovitch L

Whitmore G F

References (13)

13 references, click to expand

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Properties and uses of human-mouse hybrid cell lines.
Nature. 1968 Dec 21;220(5173):1199-202 PMID: 5725979
Mammalian cell genetics. II. Chemical induction of specific locus mutations in Chinese hamster cells in vitro.
Proc Natl Acad Sci U S A. 1968 Dec;61(4):1306-12 PMID: 5249812
The locus of action of 1-beta-d-arabinofuranosylcytosine in the cell cycle.
Cancer Res. 1969 Mar;29(3):687-96 PMID: 4180093
Sensitivity of synchronized Chinese hamster cells to ultraviolet light.
Biophys J. 1969 Sep;9(9):1171-92 PMID: 5807224
Temperature sensitive conditional mutants of monkey kidney cells.
Nature. 1969 Sep 27;223(5213):1380-1 PMID: 4980220
Actinomycin D: effects on mouse L-cells.
Biophys J. 1969 Dec;9(12):1427-45 PMID: 5357735
Genetics of somatic mammalian cells. IX. Quantitation of mutagenesis by physical and chemical agents.
J Cell Physiol. 1969 Dec;74(3):245-58 PMID: 5390857
Mammalian cell genetics. I. Selection and characterization of mutations auxotrophic for L-glutamine or resistant to 8-azaguanine in Chinese hamster cells in vitro.
Genetics. 1969 Jun;62(2):359-77 PMID: 5392645
Plaque formation and isolation of pure lines with poliomyelitis viruses.
J Exp Med. 1954 Feb;99(2):167-82 PMID: 13130792
[New observations on the in vitro hybridization of mouse cells].
C R Hebd Seances Acad Sci. 1962 Jan 3;254:181-2 PMID: 13890381
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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.66.2.377

Published

1970-06-00

Pages

377-84

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC283055

Subset

External Links

PubMed source DOI: 10.1073/pnas.66.2.377 PMC Full Text View journal details

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Isolation of temperature-sensitive mutants of L-cells.

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