Synaptogenesis in reaggregating brain cell culture.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 68 ·No. 12 ·1971-12-00 ·Pages 3219-22

Seeds NW, Vatter AE

Abstract

Dissociated cells from embryonic mouse brain reassociate in rotation culture to form highly organized aggregates. Electron microscopic observations of aggregates show the presence of synapses that mature and increase in number during culture. Apparent myelination of axons is observed in the aggregates after 5 weeks of culture. Thus, brain cell aggregates perform two highly specialized morphological events that are characteristic of mouse brain differentiation.

MeSH Terms

Animals Brain/cytology,embryology,physiology Cell Aggregation Cell Differentiation Cells, Cultured Embryo, Mammalian Female Mice Mice, Inbred Strains Microscopy, Electron Myelin Sheath/physiology Pregnancy Synapses/physiology

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Seeds N W

Vatter A E

References (12)

12 references, click to expand

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Article Info

Journal

Proceedings of the National Academy of Sciences of the United States of America

Abbr.

Proc Natl Acad Sci U S A

ISSN

0027-8424

DOI

10.1073/pnas.68.12.3219

Published

1971-12-00

Pages

3219-22

Language

English

Region

United States

NLM ID

7505876

PMCID

PMC389625

Subset

External Links

PubMed source DOI: 10.1073/pnas.68.12.3219 PMC Full Text View journal details

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