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PMID: 54443 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

In vitro transcription of 70S RNA by the RNA-directed DNA polymerase of Rouse sarcoma virus: lack of influence of RNase H.

Journal of virology ·Vol. 17 ·No. 1 ·1975-01-00 ·Pages 291-5

Collett MS, Faras AJ

Abstract

The influence of Rous sarcoma virus (RSV)-associated RNase H on the in vitro synthesis of DNA by the RSV RNA-directed DNA polymerase was determined under conditions whereby RNase H activity was selectively inhibited with NaF. Not only were we unable to detect any effect on the size, structure, or genetic complixity of the DNA product synthesized in the absence of RNase H activity, but the displacement of DNA from the 70S RNA:DNA hybrid structures was also unaffected. The suitability of 70S RNA:DNA hybrid structures synthesized in vitro to serve as a substrate for RNase H is discussed.

MeSH Terms

Avian Sarcoma Viruses/enzymology Cell-Free System DNA, Viral/biosynthesis Fluorides/pharmacology RNA, Viral/metabolism RNA-Directed DNA Polymerase/metabolism Ribonucleases/metabolism Transcription, Genetic

Chemicals

DNA, Viral RNA, Viral RNA-Directed DNA Polymerase Ribonucleases Fluorides

Authors & Affiliations

2 authors, click to expand affiliations / ORCID

Collett M S

Faras A J

References (24)

24 references, click to expand

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Article Info

Journal

Journal of virology

Abbr.

J Virol

ISSN

0022-538X

DOI

10.1128/JVI.17.1.291-295.1976

Published

1975-01-00

Pages

291-5

Language

English

Region

United States

NLM ID

0113724

PMCID

PMC515415

Subset

External Links

PubMed source DOI: 10.1128/JVI.17.1.291-295.1976 PMC Full Text View journal details

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In vitro transcription of 70S RNA by the RNA-directed DNA polymerase of Rouse sarcoma virus: lack of influence of RNase H.

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