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PMID: 565417 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Spring viremia of carp virus RNA and virion-associated transcriptase activity.

Journal of virology ·Vol. 25 ·No. 3 ·1978-03-00 ·Pages 912-6

Roy P, Clewley JP

Abstract

An RNA-dependent RNA polymerase activity has been demonstrated for spring viremia of carp virus (SVCV). The optimal temperature for in vitro synthesis of RNA was 20 to 25 degrees C. The SVCV enzyme activity was stimulated when the methyl donor S-adenosyl-L-methionine was included in the reaction mixture. S-adenosyl-L-methionine was not particularly effective in stimulating the virion RNA polymerase activity of vesicular stomatitis virus or pike fry rhabdovirus. The 5' nucleotide of the SVCV viral RNA is pppAp.

MeSH Terms
Animals Base Sequence Cell Line Fish Diseases/microbiology Oligonucleotides/analysis RNA Nucleotidyltransferases/metabolism RNA, Messenger/biosynthesis RNA, Viral/biosynthesis RNA-Dependent RNA Polymerase/metabolism Rhabdoviridae/analysis,enzymology S-Adenosylmethionine/pharmacology
Chemicals
Oligonucleotides RNA, Messenger RNA, Viral S-Adenosylmethionine RNA Nucleotidyltransferases RNA-Dependent RNA Polymerase
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Roy P
Clewley J P
References (22)
22 references, click to expand
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Article Info
Journal
Journal of virology
Abbr.
J Virol
ISSN
0022-538X
Published
1978-03-00
Pages
912-6
Language
English
Region
United States
NLM ID
0113724
PMCID
PMC525986
Subset
IM
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