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PMID: 571893 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Human megakaryocytes. I. Characterization of the membrane and cytoplasmic components of isolated marrow megakaryocytes.

The Journal of experimental medicine ·Vol. 149 ·No. 6 ·1979-06-01 ·Pages 1273-87

Rabellino EM, Nachman RL, Williams N, Winchester RJ, Ross GD

Abstract

Human marrow megakaryocytes have been isolated with high purity and yield by processing marrow cells sequentially through density centrifugation and velocity sedimentation. Analysis of the isolated cells for various platelet-associated components by immunofluorescence demonstrated that fibrinogen, plasma factor VIII antigen (factor VIII:AGN) platelet myosin, platelet glycoproteins I and III are present on the membrane and in the cytoplasm of over 90% of marrow megakaryocytes. Parallel studies of human and mouse megakaryocytes and platelets for IgG receptor (FcR), complement receptor type one (CR1) (C3b receptor), complement receptor type two (CR2) (C3d receptor), and Ia antigen by fluorescence and (or) rosette formation methods were performed. FcR were present on most human megakaryocytes and platelets. The Ia antigen was detected on a proportion (10-15%) of human megakaryocytes but it was undetectable on human platelets. CR1 was found on 20-40% of mouse megakaryocytes and also on a proportion of mouse platelets. These differentiation markers may be of use in monitoring megakaryocyte maturation.

MeSH Terms
Animals Antigens, Surface/analysis Binding Sites Blood Coagulation Factors/analysis Blood Proteins/analysis Cell Membrane/analysis Cell Separation Centrifugation, Density Gradient Complement System Proteins Cytoplasm/analysis Glycoproteins/analysis Humans Megakaryocytes/analysis,immunology Membrane Proteins/analysis Mice Myosins/analysis
Chemicals
Antigens, Surface Blood Coagulation Factors Blood Proteins Glycoproteins Membrane Proteins Complement System Proteins Myosins
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Rabellino E M
Nachman R L
Williams N
Winchester R J
Ross G D
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42 references, click to expand
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Article Info
Journal
The Journal of experimental medicine
Abbr.
J Exp Med
ISSN
0022-1007
DOI
10.1084/jem.149.6.1273
Published
1979-06-01
Pages
1273-87
Language
English
Region
United States
NLM ID
2985109R
PMCID
PMC2184888
Subset
IM
External Links
PubMed source DOI: 10.1084/jem.149.6.1273 PMC Full Text View journal details
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