The incorporation of long-chain fatty acids into phospholipids of respiring slices of rat cerebrum.

The Biochemical journal ·Vol. 102 ·No. 1 ·1967-01-00 ·Pages 373-80

Webster GR

Abstract

1. Respiring slices of adult rat cerebrum have been shown to incorporate long-chain (14)C-labelled fatty acids into phospholipid. 2. Labelling was almost entirely confined to lecithin and ethanolamine phospholipid, only traces being present in serine phospholipid. 3. Palmitic acid, oleic acid and linoleic acid were incorporated more actively into lecithin than into ethanolamine phospholipid, but the converse was found with stearic acid. 4. All four acids labelled the 1- and 2-positions of both lipids; palmitic acid, oleic acid and linoleic acid were approximately evenly distributed, but stearic acid was incorporated predominantly at the 1-position. 5. It is considered that incorporation is most likely brought about through acylation of endogenously derived lysophosphatides. 6. The possible implications of this pathway of lipid metabolism in nervous tissue are discussed.

MeSH Terms

Animals Brain/metabolism Carbon Isotopes Chromatography, Thin Layer In Vitro Techniques Linoleic Acids/metabolism Oleic Acids/metabolism Oxygen Consumption Palmitic Acids/metabolism Phospholipids/biosynthesis Rats Stearic Acids/metabolism

Chemicals

Carbon Isotopes Linoleic Acids Oleic Acids Palmitic Acids Phospholipids Stearic Acids

Authors & Affiliations

1 authors, click to expand affiliations / ORCID

Webster G R

References (28)

28 references, click to expand

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Article Info

Journal

The Biochemical journal

Abbr.

Biochem J

ISSN

0264-6021

DOI

10.1042/bj1020373

Published

1967-01-00

Pages

373-80

Language

English

Region

England

NLM ID

2984726R

PMCID

PMC1270249

Subset

External Links

PubMed source DOI: 10.1042/bj1020373 PMC Full Text View journal details

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The incorporation of long-chain fatty acids into phospholipids of respiring slices of rat cerebrum.

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