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PMID: 6090394 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, Non-P.H.S.

Physical organization of the Bradyrhizobium japonicum nitrogenase gene region.

Journal of bacteriology ·Vol. 159 ·No. 3 ·1984-09-00 ·Pages 857-62

Adams TH, McClung CR, Chelm BK

Abstract

In Bradyrhizobium japonicum USDA 110 the three genes that encode the nitrogenase enzyme complex are separated into two transcription units, nifH and nifDK. We have physically mapped a 33-kilobase-pair region of the B. japonicum genome that contains both nifH and nifDK. The nifDK operon is located transcriptionally upstream from nifH, and all three genes are transcribed in the same direction. Within the 20-kilobase-pair region that separates the promoters for these two transcription units, we have identified a region homologous to the Klebsiella pneumoniae nifA gene. This nifA homology is situated about 6 kilobase pairs upstream from the nifH transcriptional initiation signal, in an analogous position to the nifA-like locus previously described for Rhizobium meliloti. In addition, we have characterized a second distinct nifA homology in B. japonicum that is not directly linked to the nitrogenase structural gene region.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes Genes Genes, Bacterial Gram-Negative Aerobic Bacteria/enzymology,genetics Nitrogenase/genetics Nucleic Acid Hybridization Plasmids
Chemicals
Nitrogenase DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Adams T H
McClung C R
Chelm B K
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40 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.159.3.857-862.1984
Published
1984-09-00
Pages
857-62
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC215737
Subset
IM
External Links
PubMed source DOI: 10.1128/jb.159.3.857-862.1984 PMC Full Text View journal details
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