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PMID: 6092868 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Transcriptional control of polarity in Escherichia coli by cAMP.

Molecular & general genetics : MGG ·Vol. 195 ·No. 1-2 ·1984-00-00 ·Pages 96-100

Guidi-Rontani C, Danchin A, Ullmann A

Abstract

In Escherichia coli, 3'5'-adenosine cyclic monophosphate (cAMP) and its receptor protein (CAP) are known to be involved in the control of transcription initiation of catabolic operons. In previous papers we have shown that the cAMP-CAP complex is also involved as a modulator of polarity in polycistronic transcription units. Furthermore we showed that there exists a functional relationship between this complex and the transcription termination protein, Rho. In this work, we measured mRNA synthesis corresponding to the promoter proximal and distal parts of the lac and gal operons by DNA-RNA hybridization. We show that in these operons the main polarity effect is essentially transcriptional and the cAMP-CAP complex decreases polarity by interfering with premature transcription termination.

MeSH Terms
Base Sequence Cyclic AMP/metabolism DNA Restriction Enzymes Escherichia coli/genetics,metabolism Genes Genes, Bacterial Genotype Lac Operon Nucleic Acid Hybridization Plasmids Receptors, Cyclic AMP/metabolism Rho Factor/metabolism Species Specificity Transcription, Genetic
Chemicals
Receptors, Cyclic AMP Rho Factor Cyclic AMP DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Guidi-Rontani C
Danchin A
Ullmann A
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27 references, click to expand
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Article Info
Journal
Molecular & general genetics : MGG
Abbr.
Mol Gen Genet
ISSN
0026-8925
DOI
10.1007/BF00332730
Published
1984-00-00
Pages
96-100
Language
English
Region
Germany
NLM ID
0125036
Subset
IM
External Links
PubMed source DOI: 10.1007/BF00332730
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