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PMID: 6092933 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

New human transforming genes detected by a tumorigenicity assay.

Molecular and cellular biology ·Vol. 4 ·No. 9 ·1984-09-00 ·Pages 1695-705

Fasano O, Birnbaum D, Edlund L, Fogh J, Wigler M

Abstract

We have developed a sensitive bioassay for transforming genes based on the tumorigenicity of cotransfected NIH3T3 cells in nude mice. The assay differs substantially from the NIH3T3 focus assay. Using it, we have detected the transfer of three transforming genes from the DNA of MCF-7, a human mammary carcinoma cell line. One of these is N-ras, which is amplified in MCF-7 DNA. The other two, which we have called mcf2 and mcf3, do not appear to be related to known oncogenes. We cannot detect their transfer by using the NIH3T3 focus assay. We do not yet know whether either mcf2 or mcf3 is associated with genetic abnormalities in MCF-7 cells.

MeSH Terms
Animals Breast Neoplasms Cell Line Cell Transformation, Neoplastic Cells, Cultured Cloning, Molecular DNA Restriction Enzymes DNA, Neoplasm/genetics,isolation & purification Female Humans Mice Mice, Inbred Strains Mice, Nude Neoplasm Transplantation Nucleic Acid Hybridization Oncogenes Transfection Transplantation, Heterologous
Chemicals
DNA, Neoplasm DNA Restriction Enzymes
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Fasano O
Birnbaum D
Edlund L
Fogh J
Wigler M
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54 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.9.1695-1705.1984
Published
1984-09-00
Pages
1695-705
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368975
Subset
IM
External Links
PubMed source DOI: 10.1128/mcb.4.9.1695-1705.1984 PMC Full Text View journal details
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