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PMID: 6095066 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.

Structure and arrangement of the beta-tubulin genes of Leishmania tropica.

Molecular and cellular biology ·Vol. 4 ·No. 7 ·1984-07-00 ·Pages 1372-83

Huang PL, Roberts BE, Pratt DM, David JR, Miller JS

Abstract

We studied the organization and arrangement of the genes encoding beta-tubulin in the protozoan parasite Leishmania tropica and examined the structure and orientation of the beta-tubulin mRNA relative to the gene. There were found to be eight to nine beta-tubulin genes arranged in an array of direct tandem repeat units with a length of 3.8 kilobase pairs, and they were extremely homologous, if not identical, in sequence. These repeat units did not contain the alpha-tubulin genes. The transcribed sequences within the beta-tubulin genes were localized, and the orientation of the major alpha-tubulin mRNA was mapped on the gene by S1 nuclease analysis.

MeSH Terms
Animals Base Sequence Cloning, Molecular DNA/analysis DNA Restriction Enzymes Endonucleases Genes Kinetics Leishmania/genetics,metabolism Nucleic Acid Hybridization Protein Biosynthesis RNA, Messenger/genetics Single-Strand Specific DNA and RNA Endonucleases Tubulin/genetics
Chemicals
RNA, Messenger Tubulin DNA Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Huang P L
Roberts B E
Pratt D M
David J R
Miller J S
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43 references, click to expand
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Article Info
Journal
Molecular and cellular biology
Abbr.
Mol Cell Biol
ISSN
0270-7306
DOI
10.1128/mcb.4.7.1372-1383.1984
Published
1984-07-00
Pages
1372-83
Language
English
Region
United States
NLM ID
8109087
PMCID
PMC368920
Subset
IM
Grants
NCI NIH HHS · 5T32-CA09141 · United States
NIAID NIH HHS · AI 16479 · United States
External Links
PubMed source DOI: 10.1128/mcb.4.7.1372-1383.1984 PMC Full Text View journal details
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