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PMID: 6095192 Published · ppublish English Journal Article Research Support, U.S. Gov't, P.H.S.

Interaction of cyclic AMP receptor protein with the ilvB biosynthetic operon in E. coli.

Nucleic acids research ·Vol. 12 ·No. 21 ·1984-11-12 ·Pages 8145-60

Friden P, Tsui P, Okamoto K, Freundlich M

Abstract

DNase and restriction site protection studies show that cAMP and its receptor protein (CRP) bind to the promoter of the ilvB operon at approximately position -44 to -82. This region contains sequences that are homologous to those found in other CRP-dependent promoters. In vitro transcription from the ilvB promoter was markedly increased by the addition of cAMP and CRP. This stimulation was not found when the ilvB template lacked the proposed CRP binding site. cAMP-CRP did not alter the extent of transcription termination within the ilvB leader suggesting that this regulatory system may be independent of the attenuation mechanism involved in the negative control of this operon. The results of restriction enzyme site protection studies and experiments with altered promoter fragments indicate that the mechanism for CRP stimulation of the ilvB operon may be similar to a model recently proposed for lac.

MeSH Terms
Acetolactate Synthase/genetics Amino Acid Sequence Base Sequence Carrier Proteins/metabolism Cyclic AMP Receptor Protein DNA Restriction Enzymes Escherichia coli/genetics,metabolism Genes Genes, Bacterial Operon Promoter Regions, Genetic Receptors, Cyclic AMP/metabolism Transcription, Genetic
Chemicals
Carrier Proteins Cyclic AMP Receptor Protein Receptors, Cyclic AMP Acetolactate Synthase DNA Restriction Enzymes
Authors & Affiliations
4 authors, click to expand affiliations / ORCID
Friden P
Tsui P
Okamoto K
Freundlich M
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23 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.21.8145
Published
1984-11-12
Pages
8145-60
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320290
Subset
IM
Grants
NIGMS NIH HHS · GM17152 · United States
Databases
GENBANK
J01633, X01131, X02541
External Links
PubMed source DOI: 10.1093/nar/12.21.8145 PMC Full Text View journal details
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