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PMID: 6315676 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Action of CAP on the malT promoter in vitro.

Journal of bacteriology ·Vol. 156 ·No. 3 ·1983-12-00 ·Pages 1135-43

Chapon C, Kolb A

Abstract

DNase I footprinting experiments demonstrated that CAP, the cyclic AMP receptor protein of Escherichia coli, binds around position -70 at the promoter of malT, the positive regulator gene of the maltose regulon. The binding of CAP in the presence of cyclic AMP favored the subsequent specific binding of RNA polymerase. Initiation of malT transcription in vitro displayed an absolute requirement for CAP at all tested RNA polymerase concentrations. However this was not the case with a mutant promoter (malTp1), which leads to CAP-independent malT expression in vivo. In that case an effect of CAP was seen only at the lower concentrations of RNA polymerase. These results, which suggest that CAP stimulates malT expression by promoting the binding of polymerase to the promoter, are compared with those obtained in other systems.

MeSH Terms
Binding Sites Cyclic AMP/pharmacology DNA-Directed RNA Polymerases/metabolism Escherichia coli/genetics,metabolism Genes, Regulator Maltose/metabolism Mutation Operon/drug effects Receptors, Cyclic AMP/metabolism,pharmacology Transcription, Genetic
Chemicals
Receptors, Cyclic AMP Maltose Cyclic AMP DNA-Directed RNA Polymerases
Authors & Affiliations
2 authors, click to expand affiliations / ORCID
Chapon C
Kolb A
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42 references, click to expand
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Article Info
Journal
Journal of bacteriology
Abbr.
J Bacteriol
ISSN
0021-9193
DOI
10.1128/jb.156.3.1135-1143.1983
Published
1983-12-00
Pages
1135-43
Language
English
Region
United States
NLM ID
2985120R
PMCID
PMC217959
Subset
IM
Databases
GENBANK
J01647, K01329
External Links
PubMed source DOI: 10.1128/jb.156.3.1135-1143.1983 PMC Full Text View journal details
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