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PMID: 6259624 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Cyclic AMP receptor proteins interacts with lactose operator DNA.

Nucleic acids research ·Vol. 9 ·No. 2 ·1981-01-24 ·Pages 277-92

Schmitz A

Abstract

The wildtype lac promoter-operator region of E. coli was reinvestigated for cyclic AMP receptor protein (CRP) binding sites using the 'footprinting' method. Two distinct interaction sites were found, one in the -60 base pair region, which has previously been described, and a second one covering the lac operator. These two sites are similar in extent, orientation and the characteristic pattern of CRP-induced DNaseI enhanced cutting sites.

MeSH Terms
Base Composition Base Sequence Binding Sites DNA Restriction Enzymes DNA, Bacterial/metabolism Escherichia coli/metabolism Lac Operon Plasmids Protein Binding Receptors, Cyclic AMP/metabolism
Chemicals
DNA, Bacterial Receptors, Cyclic AMP DNA Restriction Enzymes
Authors & Affiliations
1 authors, click to expand affiliations / ORCID
Schmitz A
References (31)
31 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/9.2.277
Published
1981-01-24
Pages
277-92
Language
English
Region
England
NLM ID
0411011
PMCID
PMC326692
Subset
IM
External Links
PubMed source DOI: 10.1093/nar/9.2.277 PMC Full Text View journal details
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