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PMID: 6095197 Published · ppublish English Comparative Study Journal Article Research Support, Non-U.S. Gov't

Structural analysis of the genes encoding the molybdenum-iron protein of nitrogenase in the Parasponia rhizobium strain ANU289.

Nucleic acids research ·Vol. 12 ·No. 22 ·1984-11-26 ·Pages 8329-44

Weinman JJ, Fellows FF, Gresshoff PM, Shine J, Scott KF

Abstract

The genes encoding the Molybdenum-Iron protein component of nitrogenase (nifD and nifK) have been identified and fully characterised in the Parasponia Rhizobium strain ANU289. The two genes are contiguous and are separated from the gene encoding the Fe-protein component of nitrogenase (nifH) by 21 kb of DNA. We present the entire DNA sequence of the nifD and nifK genes, thus completing the characterisation of the primary structure of the nitrogenase genes in this Rhizobium strain. Comparison of the sequence preceding the transcription initiation point of nifDK with that preceding nifH reveals a consensus promoter sequence 5'-PyTGGCAPyG-4 bp-TTGC(T/A)-10 bp-3'. This consensus promoter is found preceding nif genes in both fast-growing and slow-growing Rhizobium strains and shows a structural similarity to that preceding the coordinately-regulated nif operons in the asymbiotic organism Klebsiella pneumoniae.

MeSH Terms
Base Sequence Cloning, Molecular DNA Restriction Enzymes Endonucleases Genes Genes, Bacterial Genetic Linkage Klebsiella pneumoniae/genetics Nitrogenase/genetics Nucleic Acid Hybridization Single-Strand Specific DNA and RNA Endonucleases Species Specificity
Chemicals
Nitrogenase Endonucleases DNA Restriction Enzymes Single-Strand Specific DNA and RNA Endonucleases
Authors & Affiliations
5 authors, click to expand affiliations / ORCID
Weinman J J
Fellows F F
Gresshoff P M
Shine J
Scott K F
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49 references, click to expand
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Article Info
Journal
Nucleic acids research
Abbr.
Nucleic Acids Res
ISSN
0305-1048
DOI
10.1093/nar/12.22.8329
Published
1984-11-26
Pages
8329-44
Language
English
Region
England
NLM ID
0411011
PMCID
PMC320364
Subset
IM
Databases
GENBANK
X01139
External Links
PubMed source DOI: 10.1093/nar/12.22.8329 PMC Full Text View journal details
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