Home LiteratureArticle Details
PMID: 6096851 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Synthesis in animal cells of hepatitis B surface antigen particles carrying a receptor for polymerized human serum albumin.

Proceedings of the National Academy of Sciences of the United States of America ·Vol. 81 ·No. 24 ·1984-12-00 ·Pages 7708-12

Michel ML, Pontisso P, Sobczak E, Malpièce Y, Streeck RE, Tiollais P

Abstract

A recombinant plasmid (pSVS dhfr) encoding the pre-S region and the S gene of human hepatitis B virus (HBV) and murine dihydrofolate reductase (DHFR) cDNA has been used for the transfection of Chinese hamster ovary (CHO) DHFR- cells. Selection of clones resistant to methotrexate has permitted amplification of HBV sequences and an increase in production of hepatitis B surface antigen (HBsAg). HBV-specific transcripts have been characterized. The HBsAg 22-nm particles contain a receptor for polymerized human serum albumin (pHSA) and elicit in animals the synthesis of antireceptor antibodies. This property is ascribed to a 34,000-dalton polypeptide in the particles, which is most likely encoded by the S gene and part of the pre-S region. Especially because the pHSA receptor is most abundantly present on the virion and because, in hepatitis B infection, the appearance of anti-pHSA receptor antibodies seems to be a highly reliable criterion for viral clearance, the HBsAg particles obtained may constitute a particularly efficient vaccine.

MeSH Terms
Animals Base Sequence Cell Line Cricetinae Cricetulus DNA/metabolism DNA Restriction Enzymes Female Hepatitis B Surface Antigens/genetics,isolation & purification Humans Macromolecular Substances Mice Ovary Serum Albumin/metabolism Tetrahydrofolate Dehydrogenase/genetics Transcription, Genetic Transfection
Chemicals
Hepatitis B Surface Antigens Macromolecular Substances Serum Albumin DNA Tetrahydrofolate Dehydrogenase DNA Restriction Enzymes
Authors & Affiliations
6 authors, click to expand affiliations / ORCID
Michel M L
Pontisso P
Sobczak E
Malpièce Y
Streeck R E
Tiollais P
References (33)
33 references, click to expand
  1. The gene S promoter of hepatitis B virus confers constitutive gene expression.
    Nucleic Acids Res. 1983 Jul 11;11(13):4645-54 PMID: 6306597
  2. Receptors for polymerized human serum albumin on hepatitis B virus particles detected by radioimmunoassay: changes in receptor activity in serum during acute and chronic infection.
    J Virol Methods. 1983 Mar;6(3):151-9 PMID: 6306031
  3. Signals regulating hepatitis B surface antigen transcription.
    Nature. 1983 Sep 22-28;305(5932):336-8 PMID: 6621688
  4. A polypeptide containing 55 amino acid residues coded by the pre-S region of hepatitis B virus deoxyribonucleic acid bears the receptor for polymerized human as well as chimpanzee albumins.
    Gastroenterology. 1984 May;86(5 Pt 1):910-8 PMID: 6323244
  5. An antibody which precipitates Dane particles in acute hepatitis type B: relation to receptor sites which bind polymerized human serum albumin on virus particles.
    Hepatology. 1984 Mar-Apr;4(2):220-6 PMID: 6200418
  6. Transcription of the hepatitis B surface antigen gene in cultured murine cells initiates within the presurface region.
    J Virol. 1984 May;50(2):563-71 PMID: 6200609
  7. Recombinant retroviral DNA yielding high expression of hepatitis B surface antigen.
    EMBO J. 1982;1(12):1573-8 PMID: 6327280
  8. Cleavage of structural proteins during the assembly of the head of bacteriophage T4.
    Nature. 1970 Aug 15;227(5259):680-5 PMID: 5432063
  9. Effect of tunicamycin on the synthesis of macromolecules in cultures of chick embryo fibroblasts infected with Newcastle disease virus.
    J Antibiot (Tokyo). 1971 Nov;24(11):785-94 PMID: 5168706
  10. Purification of biologically active globin messenger RNA by chromatography on oligothymidylic acid-cellulose.
    Proc Natl Acad Sci U S A. 1972 Jun;69(6):1408-12 PMID: 4504350
  11. A new technique for the assay of infectivity of human adenovirus 5 DNA.
    Virology. 1973 Apr;52(2):456-67 PMID: 4705382
  12. A new method for sequencing DNA.
    Proc Natl Acad Sci U S A. 1977 Feb;74(2):560-4 PMID: 265521
  13. RNA molecular weight determinations by gel electrophoresis under denaturing conditions, a critical reexamination.
    Biochemistry. 1977 Oct 18;16(21):4743-51 PMID: 911786
  14. Sizing and mapping of early adenovirus mRNAs by gel electrophoresis of S1 endonuclease-digested hybrids.
    Cell. 1977 Nov;12(3):721-32 PMID: 922889
  15. Regulation of simian virus 40 transcription: sensitive analysis of the RNA species present early in infections by virus or viral DNA.
    J Virol. 1979 Aug;31(2):360-9 PMID: 225559
  16. Fluorographic detection of radioactivity in polyacrylamide gels with the water-soluble fluor, sodium salicylate.
    Anal Biochem. 1979 Sep 15;98(1):132-5 PMID: 543547
  17. Purification of mouse immunoglobulin heavy-chain messenger RNAs from total myeloma tumor RNA.
    Eur J Biochem. 1980 Jun;107(2):303-14 PMID: 6772444
  18. Isolation of Chinese hamster cell mutants deficient in dihydrofolate reductase activity.
    Proc Natl Acad Sci U S A. 1980 Jul;77(7):4216-20 PMID: 6933469
  19. Excretion of hepatitis B surface antigen particles from mouse cells transformed with cloned viral DNA.
    Proc Natl Acad Sci U S A. 1980 Aug;77(8):4549-53 PMID: 6933503
  20. Hybridization of denatured RNA and small DNA fragments transferred to nitrocellulose.
    Proc Natl Acad Sci U S A. 1980 Sep;77(9):5201-5 PMID: 6159641
  21. Pyrimidine-specific chemical reactions useful for DNA sequencing.
    Nucleic Acids Res. 1980 Oct 24;8(20):4613-9 PMID: 7443522
  22. A simplified ultrasensitive silver stain for detecting proteins in polyacrylamide gels.
    Anal Biochem. 1980 Jul 1;105(2):361-3 PMID: 6161559
  23. Biology of hepatitis B virus.
    Science. 1981 Jul 24;213(4506):406-11 PMID: 6264599
  24. Organization and expression of eucaryotic split genes coding for proteins.
    Annu Rev Biochem. 1981;50:349-83 PMID: 6791577
  25. Glucocorticoids regulate expression of dihydrofolate reductase cDNA in mouse mammary tumour virus chimaeric plasmids.
    Nature. 1981 Nov 19;294(5838):228-32 PMID: 6272123
  26. Transcription of the hepatitis B surface antigen gene in mouse cells transformed with cloned viral DNA.
    J Virol. 1982 Apr;42(1):100-5 PMID: 7086959
  27. Synthesis and assembly of hepatitis B virus surface antigen particles in yeast.
    Nature. 1982 Jul 22;298(5872):347-50 PMID: 7045698
  28. Virus-associated receptors for polymerized human serum albumin in acute and in chronic hepatitis B virus infection.
    Gastroenterology. 1983 Feb;84(2):220-6 PMID: 6336707
  29. Variable protein composition of hepatitis B surface antigen from different donors.
    Virology. 1982 Dec;123(2):436-42 PMID: 6817511
  30. Structural relationships between minor and major proteins of hepatitis B surface antigen.
    J Virol. 1983 May;46(2):626-8 PMID: 6842680
  31. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  32. A hepatitis B surface antigen polypeptide (P31) with the receptor for polymerized human as well as chimpanzee albumins.
    Gastroenterology. 1983 Aug;85(2):268-74 PMID: 6305755
  33. Enhanced production of hepatitis B surface antigen in NIH 3T3 mouse fibroblasts by using extrachromosomally replicating bovine papillomavirus vector.
    Mol Cell Biol. 1983 Jun;3(6):1032-9 PMID: 6308420
Article Info
Journal
Proceedings of the National Academy of Sciences of the United States of America
Abbr.
Proc Natl Acad Sci U S A
ISSN
0027-8424
DOI
10.1073/pnas.81.24.7708
Published
1984-12-00
Pages
7708-12
Language
English
Region
United States
NLM ID
7505876
PMCID
PMC392221
Subset
IM
External Links
PubMed source DOI: 10.1073/pnas.81.24.7708 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2