Home LiteratureArticle Details
PMID: 6099661 Published · ppublish English Journal Article Research Support, Non-U.S. Gov't

Sequence of the membrane protein gene from avian coronavirus IBV.

Virus research ·Vol. 1 ·No. 4 ·1984-00-00 ·Pages 303-13

Boursnell ME, Brown TD, Binns MM

Abstract

cDNA clones prepared from genomic RNA of coronavirus IBV have been sequenced. The nucleotide sequence for the complete 5' region of mRNA C, which is not present in mRNAs A and B, has been determined. A sequence of 1224 bases is presented which contains a long open reading frame predicting a polypeptide of molecular weight 25 443. This is in agreement with the molecular weight of 23 000 reported for the unglycosylated form of the membrane polypeptide.

MeSH Terms
Base Sequence Coronaviridae/genetics Infectious bronchitis virus/genetics Molecular Weight RNA, Messenger/analysis RNA, Viral/analysis Viral Matrix Proteins Viral Proteins/genetics
Chemicals
RNA, Messenger RNA, Viral Viral Matrix Proteins Viral Proteins
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Boursnell M E
Brown T D
Binns M M
References (29)
29 references, click to expand
  1. Coronavirus proteins: biogenesis of avian infectious bronchitis virus virion proteins.
    J Virol. 1982 Dec;44(3):794-803 PMID: 6294329
  2. The biology of coronaviruses.
    J Gen Virol. 1983 Apr;64 (Pt 4):761-76 PMID: 6300299
  3. Structural polypeptides of coronavirus IBV.
    J Gen Virol. 1981 Mar;53(Pt 1):93-103 PMID: 6268743
  4. A rapid boiling method for the preparation of bacterial plasmids.
    Anal Biochem. 1981 Jun;114(1):193-7 PMID: 6269464
  5. Coronavirus multiplication: locations of genes for virion proteins on the avian infectious bronchitis virus genome.
    J Virol. 1984 Apr;50(1):22-9 PMID: 6321790
  6. Characterization of replicative intermediate RNA of mouse hepatitis virus: presence of leader RNA sequences on nascent chains.
    J Virol. 1983 Dec;48(3):633-40 PMID: 6313963
  7. The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with synthetic universal primers.
    Gene. 1982 Oct;19(3):259-68 PMID: 6295879
  8. Poliovirus type 3: molecular cloning of the genome and nucleotide sequence of the region encoding the protease and polymerase proteins.
    Nucleic Acids Res. 1983 Mar 11;11(5):1267-81 PMID: 6298739
  9. Coronavirus multiplication strategy. I. Identification and characterization of virus-specified RNA.
    J Virol. 1980 Jun;34(3):665-74 PMID: 6247505
  10. Coronavirus multiplication strategy. II. Mapping the avian infectious bronchitis virus intracellular RNA species to the genome.
    J Virol. 1980 Nov;36(2):440-9 PMID: 6253669
  11. DNA sequencing with chain-terminating inhibitors.
    Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 PMID: 271968
  12. Presence of leader sequences in the mRNA of mouse hepatitis virus.
    J Virol. 1983 Jun;46(3):1027-33 PMID: 6304334
  13. Coronavirus mRNA synthesis involves fusion of non-contiguous sequences.
    EMBO J. 1983;2(10):1839-44 PMID: 6196191
  14. Synthesis and processing of asparagine-linked oligosaccharides.
    Annu Rev Biochem. 1981;50:555-83 PMID: 7023366
  15. Improvements of DNA sequencing gels.
    Anal Biochem. 1981 Aug;115(2):450-7 PMID: 7304971
  16. Coronavirus proteins: structure and function of the oligosaccharides of the avian infectious bronchitis virus glycoproteins.
    J Virol. 1982 Dec;44(3):804-12 PMID: 6294330
  17. Nucleotide sequences of the mRNA's encoding the vesicular stomatitis virus G and M proteins determined from cDNA clones containing the complete coding regions.
    J Virol. 1981 Aug;39(2):519-28 PMID: 6268840
  18. Rapid synthesis of oligodeoxyribonucleotides. VII. Solid phase synthesis of oligodeoxyribonucleotides by a continuous flow phosphotriester method on a kieselguhr-polyamide support.
    Nucleic Acids Res. 1982 Oct 25;10(20):6243-54 PMID: 7177846
  19. Characterization of coronavirus II. Glycoproteins of the viral envelope: tryptic peptide analysis.
    Virology. 1977 Apr;77(2):650-60 PMID: 855187
  20. A simple method for displaying the hydropathic character of a protein.
    J Mol Biol. 1982 May 5;157(1):105-32 PMID: 7108955
  21. Apple II computer software for DNA and protein sequence data.
    DNA. 1983;2(1):31-5 PMID: 6687867
  22. Trans-complementable copy-number mutants of plasmid ColE1.
    Nature. 1980 Jan 10;283(5743):216-8 PMID: 7350544
  23. Mouse hepatitis virus A59: mRNA structure and genetic localization of the sequence divergence from hepatotropic strain MHV-3.
    J Virol. 1981 Sep;39(3):823-34 PMID: 6169842
  24. Structural analysis of virion proteins of the avian coronavirus infectious bronchitis virus.
    J Virol. 1982 Apr;42(1):208-19 PMID: 6283141
  25. Studies on transformation of Escherichia coli with plasmids.
    J Mol Biol. 1983 Jun 5;166(4):557-80 PMID: 6345791
  26. Isolation of coronavirus envelope glycoproteins and interaction with the viral nucleocapsid.
    J Virol. 1980 Jan;33(1):449-62 PMID: 6245243
  27. Chicken ovalbumin contains an internal signal sequence.
    Nature. 1979 Sep 13;281(5727):117-21 PMID: 471058
  28. Comparison of initiation of protein synthesis in procaryotes, eucaryotes, and organelles.
    Microbiol Rev. 1983 Mar;47(1):1-45 PMID: 6343825
  29. Coronavirus IBV glycopolypeptides: size of their polypeptide moieties and nature of their oligosaccharides.
    J Gen Virol. 1983 May;64(Pt 5):1187-91 PMID: 6302214
Article Info
Journal
Virus research
Abbr.
Virus Res
ISSN
0168-1702
DOI
10.1016/0168-1702(84)90019-4
Published
1984-00-00
Pages
303-13
Language
English
Region
Netherlands
NLM ID
8410979
PMCID
PMC7134033
Subset
IM
Databases
GENBANK
M22014
External Links
PubMed source DOI: 10.1016/0168-1702(84)90019-4 PMC Full Text View journal details
Analysis Services
Analysis Services

Contact

No. 2 Wenbo Road, Zhangqiu District, Jinan, Shandong

Qilu Normal University · Genelibs Bioinformatics Lab

750 Shunhua Rd, Jinan

2F, Bldg F, University Science Park

Tel: 0531-88819269

WeChat Official Account

Follow our WeChat subscription account for real-time updates and the latest in medical and biological research.


Business Email

E-mail: [email protected]

Copyright © 2026.Genelibs Jinan Honghui Biotechnology Co., Ltd. 鲁ICP备20007159号-2