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PMID: 6100313 Published · ppublish English Comparative Study Journal Article Research Support, U.S. Gov't, Non-P.H.S.

Comparison of the nucleotide sequence of soybean 18S rRNA with the sequences of other small-subunit rRNAs.

Journal of molecular evolution ·Vol. 21 ·No. 3 ·1984-00-00 ·Pages 259-69

Eckenrode VK, Arnold J, Meagher RB

Abstract

We present the sequence of the nuclear-encoded ribosomal small-subunit RNA from soybean. The soybean 18S rRNA sequence of 1807 nucleotides (nt) is contained in a gene family of approximately 800 closely related members per haploid genome. This sequence is compared with the ribosomal small-subunit RNAs of maize (1805 nt), yeast (1789 nt), Xenopus (1825 nt), rat (1869 nt), and Escherichia coli (1541 nt). Significant sequence homology is observed among the eukaryotic small-subunit rRNAs examined, and some sequence homology is observed between eukaryotic and prokaryotic small-subunit rRNAs. Conserved regions are found to be interspersed among highly diverged sequences. The significance of these comparisons is evaluated using computer simulation of a random sequence model. A tentative model of the secondary structure of soybean 18S rRNA is presented and discussed in the context of the functions of the various conserved regions within the sequence. On the basis of this model, the short base-paired sequences defining the four structural and functional domains of all 18S rRNAs are seen to be well conserved. The potential roles of other conserved soybean 18S rRNA sequences in protein synthesis are discussed.

MeSH Terms
Animals Base Sequence Computers DNA Restriction Enzymes Genes Macromolecular Substances Molecular Weight Nucleic Acid Conformation Plants/genetics RNA, Ribosomal/genetics Soybeans Species Specificity
Chemicals
Macromolecular Substances RNA, Ribosomal DNA Restriction Enzymes
Authors & Affiliations
3 authors, click to expand affiliations / ORCID
Eckenrode V K
Arnold J
Meagher R B
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Article Info
Journal
Journal of molecular evolution
Abbr.
J Mol Evol
ISSN
0022-2844
DOI
10.1007/BF02102358
Published
1984-00-00
Pages
259-69
Language
English
Region
Germany
NLM ID
0360051
Subset
IM
External Links
PubMed source DOI: 10.1007/BF02102358 View journal details
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